THE ROLE OF GHRELIN AND GHRELIN RECEPTORS IN POLYCYSTIC OVARY SYNDROME

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HADI NADOUSHAN H.; NORMANN R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: International Journal of Reproductive BioMedicine Year:2005 | Volume:3 | Issue:2 Page(s): 68-73

Download Full-Text

View:

334

Download:

104

Cites:

Information Journal Paper

Title

THE ROLE OF GHRELIN AND GHRELIN RECEPTORS IN POLYCYSTIC OVARY SYNDROME

Author(s)

HADI NADOUSHAN H. | NORMANN R. | Issue Writer Certificate

Keywords

PCOS

GHRELIN

GHRS

Abstract

Background: Polycystic ovary syndrome (PCOS) is related to obesity and to major metabolic alterations including both insulin resistance and beta-cell dysfunction. GHRELIN was identified as the endogenous ligand for the growth hormone secretagogue (GHS) receptor. The actions of GHRELIN are carried out through interaction with specific receptor, named GHS-R.Objective: In a case-control study, we compared the expression of GHRELIN and GHS-Rs mRNA by Quantitative Real-time PCR method in studied groups in order to determine the role of GHRELIN and GHS-Rs in pathogenesis of PCOS.Materials & Methods: Follicular fluid samples were obtained at oocyte collection from 22 patients undergoing IVF-ET as control and 11 patients were diagnosed as having PCOS. Total RNA was extracted from isolated follicular fluid cells and 2µg RNA was diluted and reverses transcribed using random primers and Superscript II. Specific primers for the GHRELIN, GHS-R1a and GHS-R1b were designed. Samples were run in triplicate on an ABI Geneamp 5700 sequence detection system. They were subjected to 40 cycles of amplification under condition 92°C- 20s and 62°C-1min using 3µl diluted cDNA (1:7), 10µl 2X SYBR green, 3µl diluted cDNA. β-actin mRNA was assayed and then normalized to total RNA measurements for each sample. Results: Age, weight and resulting pregnancies did not vary between PCOS and non-PCOS patients, whereas the BMI and serum testosterone level of PCOS were significantly higher than non-PCOS patients. Quantitative real-time RT-PCR showed that mRNA for GHRELIN and GHS-R 1b were detectable in follicular fluid cells from all patients. We failed to find mRNA for GHS-R 1a in any of follicular fluid cells. There were no significant difference in GHRELIN and GHS-R1b mRNA expression levels between PCOS and non-PCOS groups. Conclusion: Our findings indicate that GHRELIN and GHRELIN receptors may not be considered risk factors for pathogenesis of PCOS.

Cites

No record.

References

No record.

Cite

APA: Copy

HADI NADOUSHAN, H., & NORMANN, R.. (2005). THE ROLE OF GHRELIN AND GHRELIN RECEPTORS IN POLYCYSTIC OVARY SYNDROME. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 3(2), 68-73. SID. https://sid.ir/paper/295082/en

Vancouver: Copy

HADI NADOUSHAN H., NORMANN R.. THE ROLE OF GHRELIN AND GHRELIN RECEPTORS IN POLYCYSTIC OVARY SYNDROME. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2005;3(2):68-73. Available from: https://sid.ir/paper/295082/en

IEEE: Copy

H. HADI NADOUSHAN, and R. NORMANN, “THE ROLE OF GHRELIN AND GHRELIN RECEPTORS IN POLYCYSTIC OVARY SYNDROME,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 3, no. 2, pp. 68–73, 2005, [Online]. Available: https://sid.ir/paper/295082/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops