مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

335
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

220
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

IN VITRO MATURATION MEDIA, CYSTEAMINE CONCENTRATION AND GLUTATHIONE LEVEL AFFECT BLSTOCYSTS DEVELOPMENT IN MOUSE

Pages

  159-163

Abstract

 Background: Preparation of OOCYTEs is one of the critical factors that determine the developmental competence of embryos produced by in vitro fertilization (IVF).Objective: In this study, the effect of CYSTEAMINE, type of media and glutathione (GSH) level on BLASTOCYSTS development after in vitro maturation of MOUSE OOCYTEs were investigated.Materials and Methods: Premature female mice were primed with pregnant mare stimulating gonadotrophin (PMSG), and germinal vesicle (GV) stage OOCYTEs were obtained 45 hr later. GV OOCYTEs were cultured in presence of 0, 50, 100, 200 and 500 mm CYSTEAMINE in TCM199 and MEME media. After IVM, MII OOCYTEs were in vitro fertilized (IVF) and in vitro cultured (IVC) in order to observe embryo development. A group of In Vivo Ovulated (IVO) OOCYTEs after priming with PMSG and HCG also were included in this study. 5,5-Dithio-bis (2nitrobenzoic acid) DTNB-recycling protocol was used for GSH assay.Results: Rate of IVM and IVF were improved in all OOCYTEs treated with CYSTEAMINE in the two medium except 500 mm (81% MII rate in TCM and 64% MII in MEME). Rate of blastocyst in 100 mm CYSTEAMINE in TCM1199 and 200 mm in MEME was higher compared to control groups (In TCM 45% and in MEME 35%). In vivo MII and GV OOCYTEs represented the highest and lowest GSH level respectively.Conclusion: Our results revealed that the media and concentration of CYSTEAMINE can affects on IVM, IVF and rate of BLASTOCYSTS development on dose dependant manner.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    MOHAMMADI ROUSHANDEH, A., PASBAKHSH, P., ALIZADEH, Z., & HABIBI ROUDKENAR, M.. (2007). IN VITRO MATURATION MEDIA, CYSTEAMINE CONCENTRATION AND GLUTATHIONE LEVEL AFFECT BLSTOCYSTS DEVELOPMENT IN MOUSE. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 5(4), 159-163. SID. https://sid.ir/paper/295133/en

    Vancouver: Copy

    MOHAMMADI ROUSHANDEH A., PASBAKHSH P., ALIZADEH Z., HABIBI ROUDKENAR M.. IN VITRO MATURATION MEDIA, CYSTEAMINE CONCENTRATION AND GLUTATHIONE LEVEL AFFECT BLSTOCYSTS DEVELOPMENT IN MOUSE. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2007;5(4):159-163. Available from: https://sid.ir/paper/295133/en

    IEEE: Copy

    A. MOHAMMADI ROUSHANDEH, P. PASBAKHSH, Z. ALIZADEH, and M. HABIBI ROUDKENAR, “IN VITRO MATURATION MEDIA, CYSTEAMINE CONCENTRATION AND GLUTATHIONE LEVEL AFFECT BLSTOCYSTS DEVELOPMENT IN MOUSE,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 5, no. 4, pp. 159–163, 2007, [Online]. Available: https://sid.ir/paper/295133/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button