RELATIONSHIP BETWEEN SPERM DNA DAMAGE WITH SPERM PARAMETERS IN IRANIAN SUBFERTILE MEN

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ALIZADEH NILI H.; MOZDARANI H.

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Paper Information

Journal: INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE Year:2009 | Volume:7 | Issue:SUPPL 1 Page(s): 0-0

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Title

RELATIONSHIP BETWEEN SPERM DNA DAMAGE WITH SPERM PARAMETERS IN IRANIAN SUBFERTILE MEN

Author(s)

ALIZADEH NILI H. | MOZDARANI H.

Keywords

SPERM PARAMETERS

DNA INTEGRITY

SEMEN

Abstract

Introduction: Sperm DNA carries one-half of the genomic material to offspring. Thus, normal sperm genetic material is required for fertilization, embryo and fetal development and postnatal wellbeing of child. Abnormal DNA can lead to derangements in any of these processes. The abnormality or defect in the genomic material may take the form of condensation or nuclear maturity defects, DNA breaks or DNA integrity defects and sperm chromosomal aneuploidy. The aim of this study is to compare the extent of DNA damage in sperms of three groups of subfertile patients with normal individuals.Materials and Methods: Semen samples were obtained from healthy subfertile couples. Semen profiles were then performed and classified according to WHO criteria into four groups (Normal, Oligospermia, Asthenospermia and Oligoasthenospermia). The modified alkaline single-cell gel electrophoresis (comet) assay was used to assess the extent of DNA damage in semen samples. The slides were viewed using a Nikon E800 fluorescence microscope, equipped with an excitation filter of 515-560 nm and a barrier filter of 590 nm. For each individual, one thousand sperm cells were counted and classified into five groups (Comet 0 - Comet 4) based on their tail moment and to calculate total DNA damages.Results: Results obtained show high background DNA damage from normospermic individuals (~30%) however a relatively linear trend of DNA damage was observed for oligospermia, asthenospermia and oligoasthenospermia. The highest value of DNA damage was observed for oligoasthenospermia samples, which was nearly 70%. These findings showed a significant correlation with semen quality.Conclusion: Our data clearly show that DNA damage is significantly more in the sperms of subfertile patients than in normal individuals. It also shows an important relationship between semen parameter and DNA fragmentation. Comet assay, as an extremely sensitive means of monitoring DNA strand breakage in human spermatozoa, can be used to classify subfertile patients.

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APA: Copy

ALIZADEH NILI, H., & MOZDARANI, H.. (2009). RELATIONSHIP BETWEEN SPERM DNA DAMAGE WITH SPERM PARAMETERS IN IRANIAN SUBFERTILE MEN. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 7(SUPPL 1), 0-0. SID. https://sid.ir/paper/295185/en

Vancouver: Copy

ALIZADEH NILI H., MOZDARANI H.. RELATIONSHIP BETWEEN SPERM DNA DAMAGE WITH SPERM PARAMETERS IN IRANIAN SUBFERTILE MEN. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2009;7(SUPPL 1):0-0. Available from: https://sid.ir/paper/295185/en

IEEE: Copy

H. ALIZADEH NILI, and H. MOZDARANI, “RELATIONSHIP BETWEEN SPERM DNA DAMAGE WITH SPERM PARAMETERS IN IRANIAN SUBFERTILE MEN,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 7, no. SUPPL 1, pp. 0–0, 2009, [Online]. Available: https://sid.ir/paper/295185/en

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