EFFECTS OF DIFFERENT DOSES OF BONE MORPHOGENETIC PROTEIN 4 ON THE VIABILITY AND PROLIFERATION OF CCE MOUSE EMBRYONIC STEM CELLS

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MAKOULATI Z.; MOVAHEDIN MANSOUREH; FOROUZANDEH MOGHADAM M.

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Journal: International Journal of Reproductive BioMedicine Year:2009 | Volume:7 | Issue:SUPPL 2 Page(s): 0-0

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Title

EFFECTS OF DIFFERENT DOSES OF BONE MORPHOGENETIC PROTEIN 4 ON THE VIABILITY AND PROLIFERATION OF CCE MOUSE EMBRYONIC STEM CELLS

Author(s)

MAKOULATI Z. | MOVAHEDIN MANSOUREH | FOROUZANDEH MOGHADAM M.

Keywords

EMBRYONIC STEM CELL

CCE

BMP4

PROLIFERATION

VIABILITY

Abstract

Introduction: With regard to the importance of BMPs roles in the formation, development and function of various vital systems during fetal life, the aim of this study was to evaluate the effect of different doses of BMP4 on the VIABILITY and PROLIFERATION of CCE mouse embryonic stem (ES) cells.Materials and Methods: CCE ES cells were tripsinized and cell suspension was prepared. The cells were counted and cultured in 96 well microplate. Each well of this plate containing 3Χ104 cells in 20% FCS in DMEM media. The cells incubated for 1 day, washed with PBS and cultured in DMEM containing different doses of BMP4 (1, 5, 25, 50 and 100ng/ml) as experimental groups. Control group was cultured in BMP4 free medium. ES cells incubated at 37oC overnight, washed with PBS, tripsinized and cell suspension was prepared separately from each well. In order to investigate the VIABILITY and PROLIFERATION rates of CCE ES cells, staining with trypan blue and counting were done. The mean number of whole cells and living cells were considered as PROLIFERATION and survival rates respectively. Data analysis was done with ANOVA test.Results : No significant differences were found between the mean number of whole cells in the different doses (p=0.18), but the mean percent of living cells showed that BMP4 in 5 and 100 ng/ml concentration had the best and the worst effects on the VIABILITY of ES cells respectively (65.56 vs. 27.24%).Conclusion: Evaluation of PROLIFERATION and VIABILITY rates using cell count and data analysis showed that addition of 5ng/ml BMP4 increased the PROLIFERATION and VIABILITY rates of CCE ES cells whereas high doses decreased these criteria. This suggests that different doses of BMP4 signaling may have different effects on ES cells behavior.

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APA: Copy

MAKOULATI, Z., MOVAHEDIN, MANSOUREH, & FOROUZANDEH MOGHADAM, M.. (2009). EFFECTS OF DIFFERENT DOSES OF BONE MORPHOGENETIC PROTEIN 4 ON THE VIABILITY AND PROLIFERATION OF CCE MOUSE EMBRYONIC STEM CELLS. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 7(SUPPL 2), 0-0. SID. https://sid.ir/paper/295438/en

Vancouver: Copy

MAKOULATI Z., MOVAHEDIN MANSOUREH, FOROUZANDEH MOGHADAM M.. EFFECTS OF DIFFERENT DOSES OF BONE MORPHOGENETIC PROTEIN 4 ON THE VIABILITY AND PROLIFERATION OF CCE MOUSE EMBRYONIC STEM CELLS. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2009;7(SUPPL 2):0-0. Available from: https://sid.ir/paper/295438/en

IEEE: Copy

Z. MAKOULATI, MANSOUREH MOVAHEDIN, and M. FOROUZANDEH MOGHADAM, “EFFECTS OF DIFFERENT DOSES OF BONE MORPHOGENETIC PROTEIN 4 ON THE VIABILITY AND PROLIFERATION OF CCE MOUSE EMBRYONIC STEM CELLS,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 7, no. SUPPL 2, pp. 0–0, 2009, [Online]. Available: https://sid.ir/paper/295438/en

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