PARTHENOGENETIC ACTIVATION OF MOUSE OOCYTES BY HYDROSTATIC PRESSURE

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MORTAZAVI M.; AZADBAKHT M.

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Journal: International Journal of Reproductive BioMedicine Year:2011 | Volume:9 | Issue:SUPPL 2 Page(s): 32-33

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Title

PARTHENOGENETIC ACTIVATION OF MOUSE OOCYTES BY HYDROSTATIC PRESSURE

Author(s)

MORTAZAVI M. | AZADBAKHT M.

Keywords

PARTENOGENETIC ACTIVATION

HYDROSTATIC PRESSURE

MOUSE

Abstract

Introduction: Parthenogenesis is production of offspring by a female and without genetic contribution from a male and meiotic chromosome reduction. HYDROSTATIC PRESSURE can act as a mechanical stimulator that rearranges egg contents, leading to new structural or molecular combinations.Alternatively, mechanical stimulation could stimulate a mechanically-gated process, such as the opening or closing of stretch-activated ion channels. Such alterations to ion channels can lead to ionic changes. In this study, we induced the parthenogenetic activation in the MOUSE oocyte by HYDROSTATIC PRESSURE.Materials and Methods: Six to 8-week-old female NMRI mice were superovulated by an injection of 10 IU of pregnant mare's serum gonadotropin (PMSG), followed 48 hr later by an injection of 10IU of human chorionic gonadotropin (hCG) 48-52 hours apart. Metaphase II oocytes were collected from oviducts 15-18 hours after hCG injection. The oocytes transferred to T6 medium and divided into treatments. There were four treatments; oocytes subjected to 20 mmHg HYDROSTATIC PRESSURE in pressure chamber for 10, 20 and 30 min were treatments I, II, III, respectively. Oocytes without pressure exposure considered as treatment IV. Oocytes from all treatments were cultured for 72 hr and embryo development was assessed.Results: Our results showed that, the best cleavage rates were better. After 24h, the highest cleavage rates was associated with treatment II (21.4%) which were significantly different with treatments I, III and IV (10.7, 6.5 and 4.33%) respectively, (p<0.05). After 48 hr, the cleavage rates was highest in treatment II (43%) compared to treatments I, III and IV (16, 16.9 and 10.26 %) respectively, (p<0.05). After 72 hr, the cleavage rates increased in treatment II (52.79) in comparison with 48 hr (p<0.05). It was constant in treatments I, III and IV.Conclusion: It is concluded that PARTENOGENETIC ACTIVATION can be induced by HYDROSTATIC PRESSURE in a time and dose manner in MOUSE oocytes.

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APA: Copy

MORTAZAVI, M., & AZADBAKHT, M.. (2011). PARTHENOGENETIC ACTIVATION OF MOUSE OOCYTES BY HYDROSTATIC PRESSURE. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 9(SUPPL 2), 32-33. SID. https://sid.ir/paper/295719/en

Vancouver: Copy

MORTAZAVI M., AZADBAKHT M.. PARTHENOGENETIC ACTIVATION OF MOUSE OOCYTES BY HYDROSTATIC PRESSURE. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2011;9(SUPPL 2):32-33. Available from: https://sid.ir/paper/295719/en

IEEE: Copy

M. MORTAZAVI, and M. AZADBAKHT, “PARTHENOGENETIC ACTIVATION OF MOUSE OOCYTES BY HYDROSTATIC PRESSURE,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 9, no. SUPPL 2, pp. 32–33, 2011, [Online]. Available: https://sid.ir/paper/295719/en

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