EFFECTS OF SELENIUM, CALCIUM AND CALCIUM IONOPHORE ON HUMAN OOCYTES IN VITRO MATURATION IN A CHEMICALLY DEFINED MEDIUM

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MAKKI MEHRI; SABOORI ELHAM; SABBAGHI MOHAMMAD ALI; KARAMI RAHELEH; FALLAH MOHAMMAD HASSAN; PEYGHAMBARI FATEMEH; ROUSTAEI HESSAM; AHMADI ABBAS

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Journal: International Journal of Reproductive BioMedicine Year:2012 | Volume:10 | Issue:4 Page(s): 343-348

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Title

EFFECTS OF SELENIUM, CALCIUM AND CALCIUM IONOPHORE ON HUMAN OOCYTES IN VITRO MATURATION IN A CHEMICALLY DEFINED MEDIUM

Author(s)

Keywords

HUMAN OOCYTES

IN VITRO MATURATION (IVM)

SELENIUM

CALCIUM

CALCIUM IONOPHORE

Abstract

Background: In vitro maturation (IVM) of HUMAN OOCYTES is an emerging procedure quickly incorporated into the world of assisted reproductive technologies. As an effective method of in vitro maturation, several studies have reported the critical role of differentions on activating the complex process involved in both gamete maturation and fertilization.Objective: In this study, we supplemented a chemically defined medium with different combinations of SELENIUM, CALCIUM and CALCIUM ionophore concentrations to obtain the best rate of HUMAN OOCYTES maturation, survival, and fertilization.Materials and Methods: As an experimental study, Three combinations of [selenium (5 mg/ml), CALCIUM (5 mg/ml) and CALCIUM ionophore (1 mg/ml)], [selenium (10 mg/ml), CALCIUM (7 mg/ml) and CALCIUM ionophore (2 mg/ml)] and [selenium (15 mg/ml), CALCIUM (10 mg/ml) and CALCIUM ionophore (5 mg/ml)] added to the chemically defined medium and the morphology of oocytes assessed after 22-24 hours in vitro maturation of the oocytes.Results: The highest percentage of MII (meiosis II) oocytes (68%), developing beyond the morula (20.1%) and the blastocyst formation (11.1%) observed in oocytes treated with 15 mg/ml SELENIUM, 10 mg/ml CALCIUM and 5 mg/ml CALCIUM ionophore. Moreover, we showed the significant rate of survival in each three combinations after 36, 72 and 96 hours.Conclusion: Maturation and activation of oocytes may be triggered by changes in intracellular ion concentrations as second messengers in signal transduction pathways. Here, we received the highest percentage of in vitro maturation and fertilization among three combinations of SELENIUM, CALCIUM and CALCIUM ionophore treatments. Using this combination of ions beside other factors might be useful for the enrichment of the HUMAN OOCYTES IVM medium.

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APA: Copy

MAKKI, MEHRI, SABOORI, ELHAM, SABBAGHI, MOHAMMAD ALI, KARAMI, RAHELEH, FALLAH, MOHAMMAD HASSAN, PEYGHAMBARI, FATEMEH, ROUSTAEI, HESSAM, & AHMADI, ABBAS. (2012). EFFECTS OF SELENIUM, CALCIUM AND CALCIUM IONOPHORE ON HUMAN OOCYTES IN VITRO MATURATION IN A CHEMICALLY DEFINED MEDIUM. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 10(4), 343-348. SID. https://sid.ir/paper/295906/en

Vancouver: Copy

MAKKI MEHRI, SABOORI ELHAM, SABBAGHI MOHAMMAD ALI, KARAMI RAHELEH, FALLAH MOHAMMAD HASSAN, PEYGHAMBARI FATEMEH, ROUSTAEI HESSAM, AHMADI ABBAS. EFFECTS OF SELENIUM, CALCIUM AND CALCIUM IONOPHORE ON HUMAN OOCYTES IN VITRO MATURATION IN A CHEMICALLY DEFINED MEDIUM. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2012;10(4):343-348. Available from: https://sid.ir/paper/295906/en

IEEE: Copy

MEHRI MAKKI, ELHAM SABOORI, MOHAMMAD ALI SABBAGHI, RAHELEH KARAMI, MOHAMMAD HASSAN FALLAH, FATEMEH PEYGHAMBARI, HESSAM ROUSTAEI, and ABBAS AHMADI, “EFFECTS OF SELENIUM, CALCIUM AND CALCIUM IONOPHORE ON HUMAN OOCYTES IN VITRO MATURATION IN A CHEMICALLY DEFINED MEDIUM,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 10, no. 4, pp. 343–348, 2012, [Online]. Available: https://sid.ir/paper/295906/en

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