THE EFFECT OF VARIOUS CONCENTRATIONS OF MYO-INOSITOL IN CULTURE MEDIUM ON DEVELOPMENT OF BOVINE EMBRYOS

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SOLTANI LEILA; KAFILZADEH FAROKH; KARAMI SHABANKAREH HAMED

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Journal: International Journal of Reproductive BioMedicine Year:2012 | Volume:10 | Issue:5 Page(s): 409-412

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Title

THE EFFECT OF VARIOUS CONCENTRATIONS OF MYO-INOSITOL IN CULTURE MEDIUM ON DEVELOPMENT OF BOVINE EMBRYOS

Author(s)

SOLTANI LEILA | KAFILZADEH FAROKH | KARAMI SHABANKAREH HAMED | Issue Writer Certificate

Keywords

BOVINE

MYO-INOSITOL

FERTILIZATION

EMBRYO DEVELOPMENT

Abstract

Background: Progress achieved in culture media formulations have resulted led to an improvement in maintaining the mammalian embryo in culture throughout the preimplantation and pre-attachment period.Objective: The objective of this study was to evaluate the effect of various concentrations of MYO-INOSITOL during in vitro FERTILIZATION of BOVINE oocytes on subsequent EMBRYO DEVELOPMENT.Materials and Methods: BOVINE cumulus oocytes complexes (COCs) were matured in vitro at 39oC, in humidified 5% CO2 atmosphere for 22-24 h. The COCs were co-incubated with epididymal spermatozoa of post mortem bulls in modified TALP medium for 22-24hr. The FERTILIZATION medium used was: 1) TALP medium without MYO-INOSITOL (control); 2) control+0.02 g/l MYO-INOSITOL; 3) control+0.03 g/l MYO-INOSITOL; 4) control+0.04 g/l MYO-INOSITOL. Zygotes were cultured in vitro for 8 days when the ratios of in vitro EMBRYO DEVELOPMENT of the hatched blastocysts were assessed and compared with the control group (p<0.05).Results: The presence of 0.04 g/l myo-inpsitol significantly improved overall morula and blastocyst rates (46.94%) compared to control (32.19%), but there was no difference in the percentage of embryos successfully developed to the morula and blastocyst stage when different levels of MYO-INOSITOL were used (46.94, 36.36 and 37.33% respectively). The mean percentage of cleavage rate was not significantly affected by treatments.Conclusion: These results suggest that, addition of 0.04 g/l MYO-INOSITOL in TALP medium is more beneficial for subsequent BOVINE embryonic development.

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APA: Copy

SOLTANI, LEILA, KAFILZADEH, FAROKH, & KARAMI SHABANKAREH, HAMED. (2012). THE EFFECT OF VARIOUS CONCENTRATIONS OF MYO-INOSITOL IN CULTURE MEDIUM ON DEVELOPMENT OF BOVINE EMBRYOS. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 10(5), 409-412. SID. https://sid.ir/paper/295909/en

Vancouver: Copy

SOLTANI LEILA, KAFILZADEH FAROKH, KARAMI SHABANKAREH HAMED. THE EFFECT OF VARIOUS CONCENTRATIONS OF MYO-INOSITOL IN CULTURE MEDIUM ON DEVELOPMENT OF BOVINE EMBRYOS. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2012;10(5):409-412. Available from: https://sid.ir/paper/295909/en

IEEE: Copy

LEILA SOLTANI, FAROKH KAFILZADEH, and HAMED KARAMI SHABANKAREH, “THE EFFECT OF VARIOUS CONCENTRATIONS OF MYO-INOSITOL IN CULTURE MEDIUM ON DEVELOPMENT OF BOVINE EMBRYOS,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 10, no. 5, pp. 409–412, 2012, [Online]. Available: https://sid.ir/paper/295909/en

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