MELATONIN EFFECT DURING DIFFERENT MATURATION STAGES OF OOCYTE AND SUBSEQUENT EMBRYO DEVELOPMENT IN MICE

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BAHADORI MOHAMMAD HADI; GHASEMIAN FATEMEH; RAMEZANI MINA; ASGARI ZAKIEH

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Journal Paper

Paper Information

Journal: International Journal of Reproductive BioMedicine Year:2013 | Volume:11 | Issue:1 Page(s): 11-18

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Title

MELATONIN EFFECT DURING DIFFERENT MATURATION STAGES OF OOCYTE AND SUBSEQUENT EMBRYO DEVELOPMENT IN MICE

Author(s)

BAHADORI MOHAMMAD HADI | GHASEMIAN FATEMEH | RAMEZANI MINA | ASGARI ZAKIEH | Issue Writer Certificate

Keywords

IN-VITRO OOCYTE MATURATION

DEVELOPMENT

IN-VITRO FERTILIZATION

MELATONIN

CUMULUS-OOCYTE COMPLEX

Abstract

Background: It is important to protect oocytes and embryos from oxidative stress in the culture medium. MELATONIN has been shown to be a direct free radical scavenger.Objective: Effect of MELATONIN during in vitro oocyte maturation, fertilization and embryo DEVELOPMENT of mouse oocytes was evaluated.Materials and Methods: Oocytes from supper-ovulated mouse were divided to two groups: cumulus oocyte complexes (COCs, group I) and denuded COC (d-COCs, group II). The oocytes were cultured in maturation medium with different doses of MELATONIN (1×101-105 nM). The cumulus expansion and nuclear status were evaluated after 24 h of in-vitro maturation. The oocytes were used for IN-VITRO FERTILIZATION. The fertilized oocytes were cultured in medium supplemented with different doses of MELATONIN.Results: The expansion (86.79%) and maturation (80.55%) rate of COCs increased in supplemented medium with 10 nM of MELATONIN vs. control group (73.33%, p<0.006 and p<0.026), but oocytes without cumulus cells indicated higher maturation rate at higher MELATONIN doses (10 and 100 mM, 84.34% and 79.5% vs.69.33% in control group (p<0.002). Fertilization rate was higher in treated medium with 1 m of MELATONIN (93.75%, p<0.007). The rate of cleavage and blastocyst formation was promoted in medium supplemented with 10 and 100 nM of MELATONIN (92.37% and 89.36% vs.81.25% in control group, p<0.002). We observed a dose dependent response to MELATONIN treatment in this experiment.Conclusion: Exogenous MELATONIN can promote cumulus cell expansion, in vitro oocyte maturation, and embryo DEVELOPMENT. However we investigated a dose-dependent response in different stages of maturation and DEVELOPMENT. It may reflect sensitive rate of oocytes and embryos to culture conditions.

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APA: Copy

BAHADORI, MOHAMMAD HADI, GHASEMIAN, FATEMEH, RAMEZANI, MINA, & ASGARI, ZAKIEH. (2013). MELATONIN EFFECT DURING DIFFERENT MATURATION STAGES OF OOCYTE AND SUBSEQUENT EMBRYO DEVELOPMENT IN MICE. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 11(1), 11-18. SID. https://sid.ir/paper/295939/en

Vancouver: Copy

BAHADORI MOHAMMAD HADI, GHASEMIAN FATEMEH, RAMEZANI MINA, ASGARI ZAKIEH. MELATONIN EFFECT DURING DIFFERENT MATURATION STAGES OF OOCYTE AND SUBSEQUENT EMBRYO DEVELOPMENT IN MICE. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2013;11(1):11-18. Available from: https://sid.ir/paper/295939/en

IEEE: Copy

MOHAMMAD HADI BAHADORI, FATEMEH GHASEMIAN, MINA RAMEZANI, and ZAKIEH ASGARI, “MELATONIN EFFECT DURING DIFFERENT MATURATION STAGES OF OOCYTE AND SUBSEQUENT EMBRYO DEVELOPMENT IN MICE,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 11, no. 1, pp. 11–18, 2013, [Online]. Available: https://sid.ir/paper/295939/en

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