RETINOIC ACID IMPROVE GERM CELL DIFFERENTIATION FROM HUMAN EMBRYONIC STEM CELLS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

XUEMEI LIU; JING YUE; BEI XU; JUAN HU; XINLING REN; QUN LIU; GUIJIN ZHU

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: International Journal of Reproductive BioMedicine Year:2013 | Volume:11 | Issue:11 Page(s): 905-912

Download Full-Text

View:

387

Download:

Cites:

Information Journal Paper

Title

RETINOIC ACID IMPROVE GERM CELL DIFFERENTIATION FROM HUMAN EMBRYONIC STEM CELLS

Author(s)

Keywords

CELL DIFFERENTIATION

EMBRYONIC STEM CELLS

GERM CELLS

RETINOIC ACID

Abstract

Background: Creation of artificial gametes may provide a universal solution for these patients with no gametes. Stem cell technology may provide a way to obtain fully functional gametes. RETINOIC ACID (RA) can initiate meiosis. Several studies have demonstrated that RA can promote sperm cells differentiation from mouse EMBRYONIC STEM CELLS (mESCs) and other cells from human EMBRYONIC STEM CELLS (hESCs).Objective: We sought to determine whether RA could promote differentiation of GERM CELLS from hESCs.Materials and Methods: hESCs were differentiated as embryoid bodies (EBs) in suspension with all-trans RA (atRA) or without atRA for 0, 1, 3, 5 and 7 days, and then the expression of VASA, SCP3, GDF9 and TEKT1 were compared by real-time PCR. The statistical differences were evaluated by one way ANOVA.Results: The expression of germ cell-specific markers including the gonocyte marker VASA, the meiotic marker SCP3, and post meiotic markers, GDF9 and TEKT1, all increased in the presence and absence of RA as EB differentiation progressed. In addition, the expression of these markers increased an average of 9.3, 6.9, 7.2 and 11.8 fold respectively in the presence of RA, compared to the absence of RA, over 5 days differentiation.Conclusion: Our results indicate that hESCs may have the potential to differentiate to primordial GERM CELLS (PGCs) and early gametes. RA can improve GERM CELLS differentiation from hESCs.

Cites

THE EFFECTS OF SERTOLI CELLS CONDITION MEDIUM AND RETINOIC ACID ON THE NUMBER OF COLONIES OF BONE MARROW MESENCHYMAL STEM CELLS

References

No record.

Cite

APA: Copy

XUEMEI, LIU, JING, YUE, BEI, XU, JUAN, HU, XINLING, REN, QUN, LIU, & GUIJIN, ZHU. (2013). RETINOIC ACID IMPROVE GERM CELL DIFFERENTIATION FROM HUMAN EMBRYONIC STEM CELLS. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 11(11), 905-912. SID. https://sid.ir/paper/296100/en

Vancouver: Copy

XUEMEI LIU, JING YUE, BEI XU, JUAN HU, XINLING REN, QUN LIU, GUIJIN ZHU. RETINOIC ACID IMPROVE GERM CELL DIFFERENTIATION FROM HUMAN EMBRYONIC STEM CELLS. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2013;11(11):905-912. Available from: https://sid.ir/paper/296100/en

IEEE: Copy

LIU XUEMEI, YUE JING, XU BEI, HU JUAN, REN XINLING, LIU QUN, and ZHU GUIJIN, “RETINOIC ACID IMPROVE GERM CELL DIFFERENTIATION FROM HUMAN EMBRYONIC STEM CELLS,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 11, no. 11, pp. 905–912, 2013, [Online]. Available: https://sid.ir/paper/296100/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops