THE EFFECTS OF EX VIVO COLD-STORAGE ON CRYOPRESERVATION OF THE GOAT (CAPRUS HIRCUS) EPIDIDYMAL SPERM

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HOSEINZADEH SANI SEYED KAMAL ALDIN; BARATI FARID; KHAKSARY MAHABADY MAHMOUD

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Journal Paper

Paper Information

Journal: International Journal of Reproductive BioMedicine Year:2013 | Volume:11 | Issue:9 Page(s): 747-752

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Title

THE EFFECTS OF EX VIVO COLD-STORAGE ON CRYOPRESERVATION OF THE GOAT (CAPRUS HIRCUS) EPIDIDYMAL SPERM

Author(s)

HOSEINZADEH SANI SEYED KAMAL ALDIN | BARATI FARID | KHAKSARY MAHABADY MAHMOUD | Issue Writer Certificate

Keywords

EPIDIDYMAL SPERM RETRIEVAL

CRYOPRESERVATION

REFRIGERATION

COLD-STORAGE

GOAT

Abstract

Background: There are many studies focused on long or short storage time of epididymal sperm (EPS) of different species. There are limited studies on preservation or CRYOPRESERVATION of the domestic GOAT EPS.Objective: The aim of the present study was to evaluate the effects of ex vivo COLD-STORAGE on freezing of EPS from GOAT (Capra hircus).Materials and Methods: In a split-plot design the caprine testes-epididymides (40 pairs) were divided to 4 storage-time groups equally (0, 24, 48 and 72 h), then subjected to CRYOPRESERVATION using Bioxell. Sperm parameters were analyzed before and after freezing.Results: Duration of COLD-STORAGE as well as freezing at all storage-time points reduced sperm viability and progressive motility while increased sperm tail abnormalities (p<0.0001). Freezing reduced the percentage of cytoplasmic droplets (p<0.0001). The percentage of detached heads was increased at all storage-time points following freezing (p=0.0019), except at time 0 h.Conclusion: It can be concluded that cold storage [in refrigerator (4oC) for 72 h] of epididymides efficiently protected the GOAT EPS in terms of progressive motility and viability. However, COLD-STORAGE may not protect the GOAT EPS against CRYOPRESERVATION with Bioxell.

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APA: Copy

HOSEINZADEH SANI, SEYED KAMAL ALDIN, BARATI, FARID, & KHAKSARY MAHABADY, MAHMOUD. (2013). THE EFFECTS OF EX VIVO COLD-STORAGE ON CRYOPRESERVATION OF THE GOAT (CAPRUS HIRCUS) EPIDIDYMAL SPERM. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 11(9), 747-752. SID. https://sid.ir/paper/296134/en

Vancouver: Copy

HOSEINZADEH SANI SEYED KAMAL ALDIN, BARATI FARID, KHAKSARY MAHABADY MAHMOUD. THE EFFECTS OF EX VIVO COLD-STORAGE ON CRYOPRESERVATION OF THE GOAT (CAPRUS HIRCUS) EPIDIDYMAL SPERM. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2013;11(9):747-752. Available from: https://sid.ir/paper/296134/en

IEEE: Copy

SEYED KAMAL ALDIN HOSEINZADEH SANI, FARID BARATI, and MAHMOUD KHAKSARY MAHABADY, “THE EFFECTS OF EX VIVO COLD-STORAGE ON CRYOPRESERVATION OF THE GOAT (CAPRUS HIRCUS) EPIDIDYMAL SPERM,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 11, no. 9, pp. 747–752, 2013, [Online]. Available: https://sid.ir/paper/296134/en

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