FREQUENCY OF NULL ALLELE OF HUMAN LEUKOCYTE ANTIGEN-G (HLA-G) LOCUS IN SUBJECTS TO RECURRENT MISCARRIAGE

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ALIZADEH NAZILA; MOSAFERI ELNAZ; FARZADI LAYA; MAJIDI JAFAR; MONFAREDAN AMIR; YOUSEFI BAHMAN; BARADARAN BEHZAD

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Journal: International Journal of Reproductive BioMedicine Year:2016 | Volume:14 | Issue:7 Page(s): 459-464

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Title

FREQUENCY OF NULL ALLELE OF HUMAN LEUKOCYTE ANTIGEN-G (HLA-G) LOCUS IN SUBJECTS TO RECURRENT MISCARRIAGE

Author(s)

Keywords

ALLELE

HLA-G*0105N

RECURRENT MISCARRIAGE

RFLPS

Abstract

Background: Human leukocyte antigen-G (HLA-G) is a non-classical class I molecule highly expressed by extravillous cytotrophoblast cells. Due to a single base pair deletion, its function can be compensated by other isoforms. Investigating the frequency of null ALLELE in RECURRENT MISCARRIAGE (RM) subjects could be useful in understanding the relationship between frequency of this ALLELE and RM in a given population.Objective: This study aimed to determine the frequency of HLA-G*0105N null ALLELE and its potential association with down-regulation of HLA-G in subjects with RM.Materials and Methods: Western blotting was used to assess the level of HLA-G protein expression. For investigating the frequency of HLA-G*0105N null ALLELE in RM subjects, PCR-RFLP method was used. Exon 3 of HLA-G gene was amplified by polymerase chain reaction (PCR). Subsequently, PpuM-1 enzyme was employed to digest the PCR products and fragments were analyzed using gel electrophoresis.Results: Digestion using restriction enzyme showed the presence of heterozygous HLA-G*0105N null ALLELE in 10% of the test population. Western blotting results confirmed the decrease in expression of HLA-G in the placental tissue of subjects with RM compared to subjects who could give normal birth.Conclusion: The frequency of heterozygous HLA-G*0105N null ALLELE was high to some extent in subjects with RM. The mutation rate in subjects suggested that there is a significant association between RM and frequency of mutations in this ALLELE.

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APA: Copy

ALIZADEH, NAZILA, MOSAFERI, ELNAZ, FARZADI, LAYA, MAJIDI, JAFAR, MONFAREDAN, AMIR, YOUSEFI, BAHMAN, & BARADARAN, BEHZAD. (2016). FREQUENCY OF NULL ALLELE OF HUMAN LEUKOCYTE ANTIGEN-G (HLA-G) LOCUS IN SUBJECTS TO RECURRENT MISCARRIAGE. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 14(7), 459-464. SID. https://sid.ir/paper/296294/en

Vancouver: Copy

ALIZADEH NAZILA, MOSAFERI ELNAZ, FARZADI LAYA, MAJIDI JAFAR, MONFAREDAN AMIR, YOUSEFI BAHMAN, BARADARAN BEHZAD. FREQUENCY OF NULL ALLELE OF HUMAN LEUKOCYTE ANTIGEN-G (HLA-G) LOCUS IN SUBJECTS TO RECURRENT MISCARRIAGE. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2016;14(7):459-464. Available from: https://sid.ir/paper/296294/en

IEEE: Copy

NAZILA ALIZADEH, ELNAZ MOSAFERI, LAYA FARZADI, JAFAR MAJIDI, AMIR MONFAREDAN, BAHMAN YOUSEFI, and BEHZAD BARADARAN, “FREQUENCY OF NULL ALLELE OF HUMAN LEUKOCYTE ANTIGEN-G (HLA-G) LOCUS IN SUBJECTS TO RECURRENT MISCARRIAGE,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 14, no. 7, pp. 459–464, 2016, [Online]. Available: https://sid.ir/paper/296294/en

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