MUTATION ANALYSIS OF EXON1 OF BONE MORPHOGENETIC PROTEIN-15 GENE IN IRANIAN PATIENTS WITH POLYCYSTIC OVARIAN SYNDROME

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MEHDIZADEH ANAHITA; SHEIKHHA MOHAMMAD HASAN; KALANTAR SEYED MEHDI; AALI BIBI SHAHNAZ; GHANEI AZAM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: International Journal of Reproductive BioMedicine Year:2016 | Volume:14 | Issue:8 Page(s): 527-532

Download Full-Text

View:

242

Download:

Cites:

Information Journal Paper

Title

MUTATION ANALYSIS OF EXON1 OF BONE MORPHOGENETIC PROTEIN-15 GENE IN IRANIAN PATIENTS WITH POLYCYSTIC OVARIAN SYNDROME

Author(s)

Keywords

BMP-15

PCOS

MUTATION

SEQUENCING

Abstract

Background: With the prevalence of 6-10%, polycystic ovarian syndrome (PCOS) is considered the most common endocrinological disorder affecting women in their reproductive age. It has been suggested that genetic factors participate in the development of PCOS. Follicular development has been considered as one of the impaired processes in PCOS. Bone morphogenetic protein-15 (BMP-15) gene is a candidate gene in follicular development and its variants may play role in pathogenesis of PCOS.Objective: To investigate whether BMP-15 gene MUTATIONs are present in Iranian women with PCOS.Materials and Methods: In this cross-sectional study 5 ml venous blood samples was taken from 70 PCOS women referring to Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran, between January to December 2014. Genomic DNA was extracted from the blood sample by salting out method. Then a set of PCR reactions for exon1 of BMP-15 gene was performed using specific primers followed by genotyping with direct SEQUENCING.Results: Two different polymorphisms were found in the gene under study. In total 20 patients (28.6%) were heterozygote (C/G), and 2 patients (2.86%) were homozygous (G/G) for c.-9C>G in 5´UTR promoter region of BMP-15 gene (rs3810682). In addition, in the coding region of exon1, three patients (4.3%) were heterozygote (G/A) for c.A308G (rs41308602). Two PCOS patients (2.86%) appeared to have both c.-9C>G (C/G) and c.A308G (G/A) variants simultaneously.Conclusion: Our research detected two polymorphisms of BMP-15 gene among PCOS patients, indicating that even though it cannot be concluded that variants of BMP-15 gene are the principal cause of polycystic ovarian syndrome; they could be involved in pathogenic process in development of PCOS.

Cites

No record.

References

No record.

Cite

APA: Copy

MEHDIZADEH, ANAHITA, SHEIKHHA, MOHAMMAD HASAN, KALANTAR, SEYED MEHDI, AALI, BIBI SHAHNAZ, & GHANEI, AZAM. (2016). MUTATION ANALYSIS OF EXON1 OF BONE MORPHOGENETIC PROTEIN-15 GENE IN IRANIAN PATIENTS WITH POLYCYSTIC OVARIAN SYNDROME. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 14(8), 527-532. SID. https://sid.ir/paper/296311/en

Vancouver: Copy

MEHDIZADEH ANAHITA, SHEIKHHA MOHAMMAD HASAN, KALANTAR SEYED MEHDI, AALI BIBI SHAHNAZ, GHANEI AZAM. MUTATION ANALYSIS OF EXON1 OF BONE MORPHOGENETIC PROTEIN-15 GENE IN IRANIAN PATIENTS WITH POLYCYSTIC OVARIAN SYNDROME. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2016;14(8):527-532. Available from: https://sid.ir/paper/296311/en

IEEE: Copy

ANAHITA MEHDIZADEH, MOHAMMAD HASAN SHEIKHHA, SEYED MEHDI KALANTAR, BIBI SHAHNAZ AALI, and AZAM GHANEI, “MUTATION ANALYSIS OF EXON1 OF BONE MORPHOGENETIC PROTEIN-15 GENE IN IRANIAN PATIENTS WITH POLYCYSTIC OVARIAN SYNDROME,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 14, no. 8, pp. 527–532, 2016, [Online]. Available: https://sid.ir/paper/296311/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops