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Information Journal Paper

Title

Granulosa cells exposed to fibroblast growth factor 8 and 18 reveal early onset of cell growth and survival

Pages

  435-442

Abstract

 Background: Fibroblast growth factors (FGFs) are growth factors that have diverse biological activities including broad mitogenic and cell survival activities. They function through the activation of a specific tyrosine kinase receptor that transduces the signal by activating several intracellular signaling pathways. Objective: To identify the different signaling pathways involved in the mechanism of action of FGF8 and FGF18 on ovine Granulosa cells using Mass spectrometry. Materials and Methods: Ovine ovarian Granulosa cells were harvested from adult sheep independently at the stage of the estrous cycle and were cultured at a density of 500, 000 viable cells in 1 ml DMEM/F12 medium for five days. The cells were then treated on day 5 of culture with 10 ng/mL FGF8 and FGF18 for 30 minutes, and total cell protein was collected for Mass spectrometry. Results: Mass spectrometry showed that both FGF8 and FGF18 significantly induce simultaneous upregulation of several proteins, including ATF1, STAT3, MAPK1, MAPK3, MAPK14, PLCG1, PLCG2, PKCA, PIK3CA, RAF1, GAB1, and BAG2 (> 1. 5-fold; p < 0. 01). Conclusion: ATF1 and STAT3 are important transcription factors involved in cell growth, Proliferation and survival, and consequently can hamper or rescue the normal ovine reproductive system function.

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    APA: Copy

    Amin Marashi, Fatemeh, TORABI, ALI, & Beaudry, Francis. (2019). Granulosa cells exposed to fibroblast growth factor 8 and 18 reveal early onset of cell growth and survival. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 17(6), 435-442. SID. https://sid.ir/paper/296570/en

    Vancouver: Copy

    Amin Marashi Fatemeh, TORABI ALI, Beaudry Francis. Granulosa cells exposed to fibroblast growth factor 8 and 18 reveal early onset of cell growth and survival. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2019;17(6):435-442. Available from: https://sid.ir/paper/296570/en

    IEEE: Copy

    Fatemeh Amin Marashi, ALI TORABI, and Francis Beaudry, “Granulosa cells exposed to fibroblast growth factor 8 and 18 reveal early onset of cell growth and survival,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 17, no. 6, pp. 435–442, 2019, [Online]. Available: https://sid.ir/paper/296570/en

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