THE INFLUENCE OF PERFORIN EXPRESSION ON THE SENSITIVITY OF LAK/NK KILLING AGAINST VARIOUS TUMOR TARGET CELLS

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AMIR GHOFRAN Z.

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Journal: IRANIAN JOURNAL OF IMMUNOLOGY Year:2005 | Volume:2 | Issue:1 Page(s): 29-35

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Title

THE INFLUENCE OF PERFORIN EXPRESSION ON THE SENSITIVITY OF LAK/NK KILLING AGAINST VARIOUS TUMOR TARGET CELLS

Author(s)

AMIR GHOFRAN Z. | Issue Writer Certificate

Keywords

HLA

LAK/NK ACTIVITY

PERFORIN

Abstract

Background: Perforin is known to be important in cytolytic activity mediated by natural killer (NK) cells. Objective: To study the relationship between the efficiency of NK and lymphokine-activated killer (LAK) cells activity and the expression of perforin and HLAclass I molecules. Methods: LAK cells were generated by in vitro culturing of human peripheral blood lymphocytes (PBLs) in the presence of human recombinant interleukin-2 (rIL-2). Cytotoxic activity was measured at different intervals of activation by MTTcolorimetric assay using different human tumor cell lines. Immunocytochemical staining of molecules was performed on LAK/NK cells using specific monoclonal antibodies and Biotin-conjugated anti-immunoglobulin.Results: LAK/NK killing against Fen and two other cell lines, KB and Scaber showed that at day 9 and 15 of activation, 57% to 60% and 45.5% to 92.5% of Fen cells were killed at different E/Tratios. At the same time, the maximum percent killing against Scaber and KB cell lines was 47.3 and 54.3 at 5/1 ratio, respectively, showing that Fen cells were more sensitive than the two other cells. Time-course experiments using Fen cell line demonstrated 60.0, 83.9 and 34.8 percent killing at days 9, 15 and 22 at 10/1 E/Tratios. When other E/Tratios were investigated, a similar profile was observed. The maximum activity was at day 15 and 5/1 E/Tratio (92.5%). In immunocytochem-ical staining of activated LAK cells, 75.9% to 86.3% of LAK cells expressed HLA class I molecules. Perforin expression changed from 30.3% at day 7 to 42.7% at day 17 followed by a decrease to 27.9% at day 24. Conclusion: These data indicate that perforin expression is closely correlated with NK/LAK killing activity.

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APA: Copy

AMIR GHOFRAN, Z.. (2005). THE INFLUENCE OF PERFORIN EXPRESSION ON THE SENSITIVITY OF LAK/NK KILLING AGAINST VARIOUS TUMOR TARGET CELLS . IRANIAN JOURNAL OF IMMUNOLOGY (IJI), 2(1), 29-35. SID. https://sid.ir/paper/296692/en

Vancouver: Copy

AMIR GHOFRAN Z.. THE INFLUENCE OF PERFORIN EXPRESSION ON THE SENSITIVITY OF LAK/NK KILLING AGAINST VARIOUS TUMOR TARGET CELLS . IRANIAN JOURNAL OF IMMUNOLOGY (IJI)[Internet]. 2005;2(1):29-35. Available from: https://sid.ir/paper/296692/en

IEEE: Copy

Z. AMIR GHOFRAN, “THE INFLUENCE OF PERFORIN EXPRESSION ON THE SENSITIVITY OF LAK/NK KILLING AGAINST VARIOUS TUMOR TARGET CELLS ,” IRANIAN JOURNAL OF IMMUNOLOGY (IJI), vol. 2, no. 1, pp. 29–35, 2005, [Online]. Available: https://sid.ir/paper/296692/en

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