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Information Journal Paper

Title

EXPRESSION OF CXC CHEMOKINES GRO/KC AND SDF-1Α IN RAT H4 HEPATOMA CELLS IN RESPONSE TO DIFFERENT STIMULI

Pages

  54-60

Keywords

SDF-1A 
CHEMOKINE 

Abstract

 Background: It is now established that several environmental stresses cause activation of p38 MAP kinase and JNK in various cell types to produce chemokines.Objective: To investigate the expression of CXC chemokines Gro/KC and SDF-1a in rat H4 HEPATOMA cells in response to heat shock, hyper-osmolarity and oxidative stresses.Methods: HEPATOMA cells were maintained in MEM medium. Cells were subjected to different stresses [(H2O2 0.15% (w/v), manitol and NaCl (160 mM) and heat shock (42 °C for 20 minutes)]. Cells were harvested and RNA was extracted, purified and the CXC chemokine Gro/KC and SDF-1a expression was analysed by RT-PCR. cDNA was separated by gel electrophoresis on a 1% (w/v) agarose gel and visualized under a UV transilluminator. Results: Results obtained in this report showed that there was detectable but low expression of both SDF-1a and Gro/KC in H4 HEPATOMA cells. Heat shock failed to induce expression of SDF-1a and Gro/KC in H4 HEPATOMA cells of rat. Hyper-osmolarity also has not stimulated SDF-1a and Gro/KC expression. In this study we have also shown that oxidative stress did not induce expression of SDF-1a  and Gro/KC. Overall, although detection is possible but regulatory responses were not observed in H4 HEPATOMA cells. Conclusion: Several known injurious conditions cause recruitment of macrophages, neutrophils and other immune cells to the liver. Immune cells are recruited to the hepatic vasculature following local liver injury and consequent chemokine production. Our results demonstrated that failure to produce of chemokines by HEPATOMA cells may be a way to escape from immune surveillance.

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    APA: Copy

    HASANSHAHI RAVIZI, GH.H., KAZEMI ARABABADI, M., & DICKSON, A.J.. (2006). EXPRESSION OF CXC CHEMOKINES GRO/KC AND SDF-1Α IN RAT H4 HEPATOMA CELLS IN RESPONSE TO DIFFERENT STIMULI. IRANIAN JOURNAL OF IMMUNOLOGY (IJI), 3(2), 54-60. SID. https://sid.ir/paper/296745/en

    Vancouver: Copy

    HASANSHAHI RAVIZI GH.H., KAZEMI ARABABADI M., DICKSON A.J.. EXPRESSION OF CXC CHEMOKINES GRO/KC AND SDF-1Α IN RAT H4 HEPATOMA CELLS IN RESPONSE TO DIFFERENT STIMULI. IRANIAN JOURNAL OF IMMUNOLOGY (IJI)[Internet]. 2006;3(2):54-60. Available from: https://sid.ir/paper/296745/en

    IEEE: Copy

    GH.H. HASANSHAHI RAVIZI, M. KAZEMI ARABABADI, and A.J. DICKSON, “EXPRESSION OF CXC CHEMOKINES GRO/KC AND SDF-1Α IN RAT H4 HEPATOMA CELLS IN RESPONSE TO DIFFERENT STIMULI,” IRANIAN JOURNAL OF IMMUNOLOGY (IJI), vol. 3, no. 2, pp. 54–60, 2006, [Online]. Available: https://sid.ir/paper/296745/en

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