TYPING OF HLA CLASS I BY POLYMERASE CHAIN REACTION-SEQUENCE SPECIFIC OLIGONUCLEOTIDE PRIMER (PCR-SSOP) TECHNIQUE IN IRANIAN CORD BLOOD DONORS

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GHASHGHAIE MANSOUR A.; GHAFARI S.H.A.; ALI MOGHADAM K.; GHAVAMZADEH ARDESHIR

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Journal Paper

Paper Information

Journal: Iranian Journal of Immunology Year:2006 | Volume:3 | Issue:2 Page(s): 95-98

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Title

TYPING OF HLA CLASS I BY POLYMERASE CHAIN REACTION-SEQUENCE SPECIFIC OLIGONUCLEOTIDE PRIMER (PCR-SSOP) TECHNIQUE IN IRANIAN CORD BLOOD DONORS

Author(s)

GHASHGHAIE MANSOUR A. | GHAFARI S.H.A. | ALI MOGHADAM K. | GHAVAMZADEH ARDESHIR | Issue Writer Certificate

Keywords

HLA TYPING

PCR-SSOP

CORD BLOOD

HLA CLASS I

Abstract

Background: HLA compatibility between the donor and the recipient is one of the major determinants of transplant outcome. Therefore, HLA TYPING plays an important role in organ transplantation.Objective: 142 CORD BLOOD samples, which were registered at the CORD BLOOD bank of Iran at HORC-BMT center, were subjected to low-resolution DNA typing for HLA-A, -B and -C. Methods: Genomic DNA was prepared and HLA CLASS I was determined by the Sequence-specific Oligonucleotide Probes (SSOP-PCR). Results: A total of 284 HLA-A alleles were identified of which, A*02 and A*24 were the most common. Among 284 HLA-B and HLA-C alleles, B*35, B*51, Cw*4 and Cw*12 were the most frequent alleles in the studied population. Conclusion: Amplification of HLA loci with PCR-SSOP has proved to be a rapid and accurate method for HLA-A, -B and –C genotyping.

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APA: Copy

GHASHGHAIE MANSOUR, A., GHAFARI, S.H.A., ALI MOGHADAM, K., & GHAVAMZADEH, ARDESHIR. (2006). TYPING OF HLA CLASS I BY POLYMERASE CHAIN REACTION-SEQUENCE SPECIFIC OLIGONUCLEOTIDE PRIMER (PCR-SSOP) TECHNIQUE IN IRANIAN CORD BLOOD DONORS. IRANIAN JOURNAL OF IMMUNOLOGY (IJI), 3(2), 95-98. SID. https://sid.ir/paper/296751/en

Vancouver: Copy

GHASHGHAIE MANSOUR A., GHAFARI S.H.A., ALI MOGHADAM K., GHAVAMZADEH ARDESHIR. TYPING OF HLA CLASS I BY POLYMERASE CHAIN REACTION-SEQUENCE SPECIFIC OLIGONUCLEOTIDE PRIMER (PCR-SSOP) TECHNIQUE IN IRANIAN CORD BLOOD DONORS. IRANIAN JOURNAL OF IMMUNOLOGY (IJI)[Internet]. 2006;3(2):95-98. Available from: https://sid.ir/paper/296751/en

IEEE: Copy

A. GHASHGHAIE MANSOUR, S.H.A. GHAFARI, K. ALI MOGHADAM, and ARDESHIR GHAVAMZADEH, “TYPING OF HLA CLASS I BY POLYMERASE CHAIN REACTION-SEQUENCE SPECIFIC OLIGONUCLEOTIDE PRIMER (PCR-SSOP) TECHNIQUE IN IRANIAN CORD BLOOD DONORS,” IRANIAN JOURNAL OF IMMUNOLOGY (IJI), vol. 3, no. 2, pp. 95–98, 2006, [Online]. Available: https://sid.ir/paper/296751/en

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