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Title

INFLUENCE OF 1 ALPHA, 25-DIHYDROXYVITAMIN D3 ON T HELPER 17 CELLS AND RELATED CYTOKINES IN SYSTEMIC LUPUS ERYTHEMATOSUS

Pages

  82-93

Abstract

 Background: SYSTEMIC LUPUS ERYTHEMATOSUS (SLE) is a multisystem autoimmune disease. Emerging data suggests that T helper 17 (Th17) cells play a pathogenic role in SLE and the increased number of these cells correlates with disease activity. In recent years, 1a, 25-dihydroxyvitamin D3 (1, 25VitD3) has been considered as an immunomodulatory factor.Objective: To investigate the effect of 1, 25VitD3 on TH17 cells and on the expression of related cytokines in SLE patients.Method: Thirty SLE patients (newly diagnosed or in remission) were sampled for 10 ml whole blood to isolate peripheral blood mononuclear cells (PBMCs) using Ficoll-Hypaque density gradient centrifugation. Isolated cells were cultured in the presence and absence of 50 nM 1, 25VitD3. After incubation, cells were harvested and stimulated for 4-5 hours with phorbol myristate acetate (PMA) and ionomycin in the presence of brefeldin A. IL-17 secreting cells were analyzed by flowcytometry. RNA was extracted from cultured cells, cDNA was synthesized, and the expression levels of IL-6, IL-17, IL-23 and TGF-bgenes were assessed by real-time PCR.Results: The percentage of TH17 cells (CD3+CD8- IL-17+T cells) decreased significantly in 1, 25VitD3-treated cells (3.67 ±2.43%) compared to untreated cells (4.65 ± 2.75%) (p=0.003). The expression of TGF-bup regulated (1.38-fold) and the expression of IL-6 (50%), IL-17 (27%) and IL-23 (64%) down regulated after 1, 25VitD3 treatment.Conclusion: This study showed that 1, 25VitD3 modulates TH17 related pathways in SLE patients and revealed the immunomodulatory effect of 1, 25VitD3 on the TH17 mediated autoimmunity.

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    APA: Copy

    REIHANI, HADI, RASTIN, MARYAM, MAHMOUDI, MAHMOUD, GHORYANI, MOHSEN, ABDOLLAHI, NAFISEH, TABASI, NAFISEH SADAT, ZAMANI TAGHIZADE RABE, SHAHRZAD, & SAHEBARI, MARYAM. (2015). INFLUENCE OF 1 ALPHA, 25-DIHYDROXYVITAMIN D3 ON T HELPER 17 CELLS AND RELATED CYTOKINES IN SYSTEMIC LUPUS ERYTHEMATOSUS. IRANIAN JOURNAL OF IMMUNOLOGY (IJI), 12(2), 82-93. SID. https://sid.ir/paper/296880/en

    Vancouver: Copy

    REIHANI HADI, RASTIN MARYAM, MAHMOUDI MAHMOUD, GHORYANI MOHSEN, ABDOLLAHI NAFISEH, TABASI NAFISEH SADAT, ZAMANI TAGHIZADE RABE SHAHRZAD, SAHEBARI MARYAM. INFLUENCE OF 1 ALPHA, 25-DIHYDROXYVITAMIN D3 ON T HELPER 17 CELLS AND RELATED CYTOKINES IN SYSTEMIC LUPUS ERYTHEMATOSUS. IRANIAN JOURNAL OF IMMUNOLOGY (IJI)[Internet]. 2015;12(2):82-93. Available from: https://sid.ir/paper/296880/en

    IEEE: Copy

    HADI REIHANI, MARYAM RASTIN, MAHMOUD MAHMOUDI, MOHSEN GHORYANI, NAFISEH ABDOLLAHI, NAFISEH SADAT TABASI, SHAHRZAD ZAMANI TAGHIZADE RABE, and MARYAM SAHEBARI, “INFLUENCE OF 1 ALPHA, 25-DIHYDROXYVITAMIN D3 ON T HELPER 17 CELLS AND RELATED CYTOKINES IN SYSTEMIC LUPUS ERYTHEMATOSUS,” IRANIAN JOURNAL OF IMMUNOLOGY (IJI), vol. 12, no. 2, pp. 82–93, 2015, [Online]. Available: https://sid.ir/paper/296880/en

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