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Information Journal Paper

Title

Anti-inflammatory Effects of PMX205 in Mouse Macrophage Periodontitis Model

Pages

  84-96

Abstract

 Background: C5areceptor antagonistPMX205 is a synthetic hexapeptidecapable of blocking C5a-C5a receptor (C5aR) axis by simulating C5a active C-terminal amino acid residues. This hexapeptide presents good anti-inflammatory effects in a myriad inflammation models. The anti-inflammatory effect of PMX205 on periodontitis is yet to be fully fathomed. Objective: To examine the anti-inflammatory effects of PMX205 on RAW264. 7 murine Macrophages exposed togingipain extracts and Porphyromonas Gingivalis (P. gingivalis). Methods: MTT assay was carried out so as to specify the cytotoxicity of PMX205. RAW264. 7 cells were co-cultured in vitro with gingipain extracts or P. gingivalis to simulate the periodontitis inflammatory milieu. Real-time quantitative PCR, ELISA and Griess assay were performed in order to detect tumor necrosis factor-α (TNF-α ), IL-6, IL-23, nitric oxide (NO), IL-10, transforming growth factor-β 1 (TGF-β 1), andarginase-1 (Arg-1). Furthermore, phagocytosis assay was done to evaluate the phagocytic capacity of RAW 264. 7 cells. Finally, western blot analysis was conducted to evaluate myeloid differentiation factor 88 (MyD88). Results: PMX205 increased the expression levels of bacteriostatic substances (NO and IL-23) and anti-inflammatory cytokines (TGF-β 1, IL-10 and Arg-1); however, it reduced the expression levels of proinflammatory cytokines TNF-α and IL-6once RAW 264. 7 Macrophages were stimulated via gingipain extracts or P. gingivalis. In addition, PMX205 promoted the Macrophage phagocytosis and down-regulated protein expression of MyD88. Conclusion: PMX205 has recognizable anti-inflammatory effects in RAW 264. 7 cell inflammation model, a finding which probably opens doors to future investigations on new targets for the prevention and treatment of chronic periodontitis.

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    APA: Copy

    Li, Gege, Pan, Jiahui, Tang, Qiuling, Liu, Xinchan, Wang, Liuran, Meng, Yang, & Yu, Weixian. (2018). Anti-inflammatory Effects of PMX205 in Mouse Macrophage Periodontitis Model. IRANIAN JOURNAL OF IMMUNOLOGY (IJI), 15(2), 84-96. SID. https://sid.ir/paper/296945/en

    Vancouver: Copy

    Li Gege, Pan Jiahui, Tang Qiuling, Liu Xinchan, Wang Liuran, Meng Yang, Yu Weixian. Anti-inflammatory Effects of PMX205 in Mouse Macrophage Periodontitis Model. IRANIAN JOURNAL OF IMMUNOLOGY (IJI)[Internet]. 2018;15(2):84-96. Available from: https://sid.ir/paper/296945/en

    IEEE: Copy

    Gege Li, Jiahui Pan, Qiuling Tang, Xinchan Liu, Liuran Wang, Yang Meng, and Weixian Yu, “Anti-inflammatory Effects of PMX205 in Mouse Macrophage Periodontitis Model,” IRANIAN JOURNAL OF IMMUNOLOGY (IJI), vol. 15, no. 2, pp. 84–96, 2018, [Online]. Available: https://sid.ir/paper/296945/en

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