Comparison of Antimicrobial Effects of Triple Antibiotic Paste and Calcium Hydroxide Mixed with 2% Chlorhexidine as Intracanal Medicaments Against Enterococcus faecalis Biofilm

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GHABRAEI SHOLEH; BOLHARI BEHNAM; Marvi Sabbagh Mohammad; SOBHI AFSHAR MAHSA

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Journal Paper

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Journal: Frontiers in Dentistry Year:2018 | Volume:15 | Issue:3 Page(s): 151-160

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Title

Comparison of Antimicrobial Effects of Triple Antibiotic Paste and Calcium Hydroxide Mixed with 2% Chlorhexidine as Intracanal Medicaments Against Enterococcus faecalis Biofilm

Author(s)

GHABRAEI SHOLEH | BOLHARI BEHNAM | Marvi Sabbagh Mohammad | SOBHI AFSHAR MAHSA | Issue Writer Certificate

Keywords

Doxycycline

Metronidazole

Ciprofloxacin

Calcium Hydroxide

Chlorhexidine

Enterococcus Faecalis

Abstract

Objectives: The purpose of this in-vitro study was to determine and compare the shortest period needed for a triple antibiotic paste (TAP) and Calcium Hydroxide (Ca(OH)2) plus 2% Chlorhexidine (CHX) to eradicate the biofilm of Enterococcus Faecalis (EF) from the root canal system. Materials and Methods: Sixty-five extracted single-rooted human teeth with straight root canals were selected. The crowns were cut from the cementoenamel junction (CEJ), and canal preparations were done by step-back technique. The smear layer was removed by 17% ethylenediaminetetraacetic acid (EDTA) and 5. 25% sodium hypochlorite (NaOCl). Afterwards, the samples were sterilized with gamma ray and were placed inside microtubes for one week. During this week, the teeth were infected with EF. Then, a TAP and Ca(OH)2 mixed with 2% CHX were inserted into the canals. The roots were cut longitudinally, and dentin chips were collected from the apical part of the roots by a round bur to the depth of 400 μ m. The vital bacterial load was assessed by counting the numbers of colony-forming units (CFUs). Results: The paste of Ca(OH)2 mixed with 2% CHX was able to eradicate the EF biofilm in three days. The TAP was able to eradicate the biofilm of EF in seven days. Conclusions: It seems that Ca(OH)2 mixed with 2% CHX is more potent than the TAP against EF biofilm.

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APA: Copy

GHABRAEI, SHOLEH, BOLHARI, BEHNAM, Marvi Sabbagh, Mohammad, & SOBHI AFSHAR, MAHSA. (2018). Comparison of Antimicrobial Effects of Triple Antibiotic Paste and Calcium Hydroxide Mixed with 2% Chlorhexidine as Intracanal Medicaments Against Enterococcus faecalis Biofilm. FRONTIERS IN DENTISTRY (JOURNAL OF DENTISTRY OF TEHRAN UNIVERSITY OF MEDICAL SCIENCES), 15(3), 151-160. SID. https://sid.ir/paper/298149/en

Vancouver: Copy

GHABRAEI SHOLEH, BOLHARI BEHNAM, Marvi Sabbagh Mohammad, SOBHI AFSHAR MAHSA. Comparison of Antimicrobial Effects of Triple Antibiotic Paste and Calcium Hydroxide Mixed with 2% Chlorhexidine as Intracanal Medicaments Against Enterococcus faecalis Biofilm. FRONTIERS IN DENTISTRY (JOURNAL OF DENTISTRY OF TEHRAN UNIVERSITY OF MEDICAL SCIENCES)[Internet]. 2018;15(3):151-160. Available from: https://sid.ir/paper/298149/en

IEEE: Copy

SHOLEH GHABRAEI, BEHNAM BOLHARI, Mohammad Marvi Sabbagh, and MAHSA SOBHI AFSHAR, “Comparison of Antimicrobial Effects of Triple Antibiotic Paste and Calcium Hydroxide Mixed with 2% Chlorhexidine as Intracanal Medicaments Against Enterococcus faecalis Biofilm,” FRONTIERS IN DENTISTRY (JOURNAL OF DENTISTRY OF TEHRAN UNIVERSITY OF MEDICAL SCIENCES), vol. 15, no. 3, pp. 151–160, 2018, [Online]. Available: https://sid.ir/paper/298149/en

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