مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Cites:

1

Information Journal Paper

Title

A QUICK AND SENSITIVE LIQUID CHROMATOGRAPHY–TANDEM MASS SPECTROMETRY (LC-MS) METHOD FOR THE DETERMINATION OF ENALAPRIL AND ENALAPRILAT IN HUMAN PLASMA: APPLICATION TO A BIOEQUIVALENCE STUDY

Pages

  21-34

Abstract

 A rapid and sensitive liquid chromatography–tandem mass spectrometry (LC-MS) method was developed for the estimation of ENALAPRIL and ENALAPRILat in HUMAN PLASMA. Detection of analytes was achieved by tandem mass spectrometry with electrospray ionization (ESI) interface in positive ion mode was operated under the multiple-reaction monitoring mode. Sample pretreatment involved in a one-step protein precipitation (PPT) with percholoric acid (HClO4) of 0.15ml plasma. The reconstituted samples were chromatographed on C18 column by pumping methanol: water: acid formic74: 24: 2 (v/v) at a flow rate of 0.2 mL/min. Each plasma sample was chromatographed within 1.25 min. The standard curves were found to be linear in the range of 0.1–20 ng/mL for ENALAPRIL and ENALAPRILat with mean correlation coefficient of ³0.999 for each analyte. The intra-day and inter-day precision and accuracy results were well within the acceptable limits. The limit of quantification (LOQ) was 0.1 ng/ml for ENALAPRIL and ENALAPRILat. The mean (SD) Cmax, Tmax, AUC0-¥tand AUC0-¥ values of ENALAPRIL versus ENALAPRILat after administration of the 10 mg ENALAPRIL are 141.33 (3.51) versus73.33 (5.03) ng/mL, 1.15 (1.45) versus 4.12 (1.74) hours, 142.57 (34.34) versus 425.94 (13.09) ng/mL/h, and 150.74 (16.69) versus 455.80 (65.11) ng/mL/h respectively. The mean (SD) t1/2 was 2.72 (2.01) hours for the ENALAPRIL and 6.34 (2.13) hours for the ENALAPRILat. The developed assay method was successfully applied to a pharmacokinetic study in human male volunteers.

Cites

References

Cite

APA: Copy

DANAFAR, HOSSEIN, & HAMIDI, MEHRDAD. (2016). A QUICK AND SENSITIVE LIQUID CHROMATOGRAPHY–TANDEM MASS SPECTROMETRY (LC-MS) METHOD FOR THE DETERMINATION OF ENALAPRIL AND ENALAPRILAT IN HUMAN PLASMA: APPLICATION TO A BIOEQUIVALENCE STUDY. IRANIAN JOURNAL OF PHARMACEUTICAL SCIENCES, 12(1), 21-34. SID. https://sid.ir/paper/300344/en

Vancouver: Copy

DANAFAR HOSSEIN, HAMIDI MEHRDAD. A QUICK AND SENSITIVE LIQUID CHROMATOGRAPHY–TANDEM MASS SPECTROMETRY (LC-MS) METHOD FOR THE DETERMINATION OF ENALAPRIL AND ENALAPRILAT IN HUMAN PLASMA: APPLICATION TO A BIOEQUIVALENCE STUDY. IRANIAN JOURNAL OF PHARMACEUTICAL SCIENCES[Internet]. 2016;12(1):21-34. Available from: https://sid.ir/paper/300344/en

IEEE: Copy

HOSSEIN DANAFAR, and MEHRDAD HAMIDI, “A QUICK AND SENSITIVE LIQUID CHROMATOGRAPHY–TANDEM MASS SPECTROMETRY (LC-MS) METHOD FOR THE DETERMINATION OF ENALAPRIL AND ENALAPRILAT IN HUMAN PLASMA: APPLICATION TO A BIOEQUIVALENCE STUDY,” IRANIAN JOURNAL OF PHARMACEUTICAL SCIENCES, vol. 12, no. 1, pp. 21–34, 2016, [Online]. Available: https://sid.ir/paper/300344/en

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