In Vitro Cytotoxicity and Setting Time Assessment of Calcium-Enriched Mixture Cement, Retro Mineral Trioxide Aggregate and Mineral Trioxide Aggregate

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Pornamazeh Tahereh; YADEGARI ZAHRA; GHASEMI AMIR; Sheykh al Eslamian Seyedeh Mahsa; SHOJAEIAN SHIVA

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Journal: Iranian Endodontic Journal Year:2017 | Volume:12 | Issue:4 Page(s): 488-492

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Title

In Vitro Cytotoxicity and Setting Time Assessment of Calcium-Enriched Mixture Cement, Retro Mineral Trioxide Aggregate and Mineral Trioxide Aggregate

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Keywords

Biocompatibility

Calcium-Enriched Mixture

Mineral Trioxide Aggregate

Retro MTA

Setting Time

Abstract

Introduction: The present study sought to evaluate and compare Biocompatibility and Setting Time of Retro Mineral Trioxide Aggregate (MTA), Calcium-Enriched Mixture (CEM) and Angelus MTA. Methods and Materials: CEM cement, Angelus MTA and Retro MTA were assessed in set and fresh states. Extracts transformed to each cavity of three 24-well plates in which 1×104 cell were seeded into each well 24 h earlier. All specimens were incubated in a humidified incubator with 5% CO2 at 37° C. Mosmann’ s tetrazolium toxicity (MTT) assay was used to determine in vitro cytotoxicity on L929 mouse fibroblast cell line. Cell viability was determined at 1, 24, and 72 h after exposure. The initial Setting Time was measured by 113. 4 g Gilmore needle testing. Then, final Setting Times were assessed by the 456. 5 g Gilmore needle. Data comparisons were performed using the analysis of variance (ANOVA) and Tukey's post hoc test (α =0. 05). Results: All groups in both forms indicated higher cell vitality compared to positive control group (P<0. 001). After 24 h, the set Retro MTA showed better Biocompatibility compared to set CEM and set Angelus MTA (P<0. 001). Retro MTA showed significantly lower initial and final Setting Time compared to CEM and Angelus MTA (P<0. 001). Conclusion: Our results indicated the good cell viability values of Retro MTA and relatively short period of Setting Time. It seems a promising alternative material in clinical situations where accelerated setting is required. However, more clinical and in vivo investigations are needed for a clear decision making.

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APA: Copy

Pornamazeh, Tahereh, YADEGARI, ZAHRA, GHASEMI, AMIR, Sheykh al Eslamian, Seyedeh Mahsa, & SHOJAEIAN, SHIVA. (2017). In Vitro Cytotoxicity and Setting Time Assessment of Calcium-Enriched Mixture Cement, Retro Mineral Trioxide Aggregate and Mineral Trioxide Aggregate. IRANIAN ENDODONTIC JOURNAL (IEJ), 12(4), 488-492. SID. https://sid.ir/paper/303089/en

Vancouver: Copy

Pornamazeh Tahereh, YADEGARI ZAHRA, GHASEMI AMIR, Sheykh al Eslamian Seyedeh Mahsa, SHOJAEIAN SHIVA. In Vitro Cytotoxicity and Setting Time Assessment of Calcium-Enriched Mixture Cement, Retro Mineral Trioxide Aggregate and Mineral Trioxide Aggregate. IRANIAN ENDODONTIC JOURNAL (IEJ)[Internet]. 2017;12(4):488-492. Available from: https://sid.ir/paper/303089/en

IEEE: Copy

Tahereh Pornamazeh, ZAHRA YADEGARI, AMIR GHASEMI, Seyedeh Mahsa Sheykh al Eslamian, and SHIVA SHOJAEIAN, “In Vitro Cytotoxicity and Setting Time Assessment of Calcium-Enriched Mixture Cement, Retro Mineral Trioxide Aggregate and Mineral Trioxide Aggregate,” IRANIAN ENDODONTIC JOURNAL (IEJ), vol. 12, no. 4, pp. 488–492, 2017, [Online]. Available: https://sid.ir/paper/303089/en

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