EVALUATION OF DOT-ELISA METHOD USING EXCRETORY-SECRETARY ANTIGENS OF FASCIOLA HEPATICA IN LABORATORY DIAGNOSIS OF HUMAN FASCIOLOSIS

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ROKNI M.B.; SAMANI A.; MASOUD J.; BABAEI NASR M.

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Paper Information

Journal: IRANIAN JOURNAL OF PARASITOLOGY Year:2006 | Volume:1 | Issue:1 Page(s): 26-30

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Title

EVALUATION OF DOT-ELISA METHOD USING EXCRETORY-SECRETARY ANTIGENS OF FASCIOLA HEPATICA IN LABORATORY DIAGNOSIS OF HUMAN FASCIOLOSIS

Author(s)

ROKNI M.B. | SAMANI A. | MASOUD J. | BABAEI NASR M. | Issue Writer Certificate

Keywords

DOT-ELISA

EXCRETORY-SECRETORY ANTIGEN

FASCIOLA HEPATICA

DIAGNOSIS

Abstract

Fasciolosis DIAGNOSIS, due to low sensitivity of coprological diagnostic method has been challenging for a long period. In this study, DOT-ELISA, one of the simplest and the most sensitive tests in this regard, was evaluated using EXCRETORY-SECRETORY ANTIGENs of FASCIOLA HEPATICA to diagnose human fasciolosis Three groups consisting of patients infected with fasciolosis (n= 95), patients with other parasitic diseases (n= 37) and healthy individuals (n= 40), were implicated in the test. All collected sera were tested by Dot ELISA using EXCRETORY-SECRETORY ANTIGENs. Optimal criteria were detected as 1.5 mg of antigen per dot, serum dilution of 1:320, and anti human IgG conjugate dilution of 1:500. The sensitivity, specificity, positive and negative predictive values were 96.8%, 96.1%, 96.8% and 96.1%, respectively. In conclusion, DOT-ELISA using excretory- secretory antigens could be regarded as a cheap, rapid, antigen and serum conservative diagnostic method in diagnosing fasciolosis.

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APA: Copy

ROKNI, M.B., SAMANI, A., MASOUD, J., & BABAEI NASR, M.. (2006). EVALUATION OF DOT-ELISA METHOD USING EXCRETORY-SECRETARY ANTIGENS OF FASCIOLA HEPATICA IN LABORATORY DIAGNOSIS OF HUMAN FASCIOLOSIS. IRANIAN JOURNAL OF PARASITOLOGY, 1(1), 26-30. SID. https://sid.ir/paper/303783/en

Vancouver: Copy

ROKNI M.B., SAMANI A., MASOUD J., BABAEI NASR M.. EVALUATION OF DOT-ELISA METHOD USING EXCRETORY-SECRETARY ANTIGENS OF FASCIOLA HEPATICA IN LABORATORY DIAGNOSIS OF HUMAN FASCIOLOSIS. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2006;1(1):26-30. Available from: https://sid.ir/paper/303783/en

IEEE: Copy

M.B. ROKNI, A. SAMANI, J. MASOUD, and M. BABAEI NASR, “EVALUATION OF DOT-ELISA METHOD USING EXCRETORY-SECRETARY ANTIGENS OF FASCIOLA HEPATICA IN LABORATORY DIAGNOSIS OF HUMAN FASCIOLOSIS,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 1, no. 1, pp. 26–30, 2006, [Online]. Available: https://sid.ir/paper/303783/en

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