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Information Journal Paper

Title

SEQUENCE DIVERSITY IN TRNA GENE LOCUS A-L AMONG IRANIAN ISOLATES OF ENTAMOEBA DISPAR

Pages

  97-103

Abstract

 Background: A number of methods for detecting DIVERSITY in Entamoeba have been described over the years. In the present study the genetic polymorphism of noncoding LOCUS A-L was analyzed using PCR and sequencing in order to clarify the genotypic differences among E. dispar isolates.Methods: A total of 28 E. dispar from patients with gastrointestinal symptoms were determined and the genomic DNA was extracted directly from stool. For genotype analysis; LOCUS A-L was amplified by PCR and PCR products were sequenced. The sequences obtained were edited manually and aligned using Gene Runner software.Results: With sequencing of PCR products a reliable genetic DIVERSITY in size, number and position of the repeat units were observed among the IRANian E. dispar isolates in LOCUS A-L gene. Sequences showed variation in length from 448bp to 507bp and seven distinct types were identified.Conclusion: The genetic DIVERSITY of loci like A-L shows them to be suitable for epidemiological studies such as the characterization of the routes of transmission of these parasites in IRAN.

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    APA: Copy

    NAZEMALHOSSEINI MOJARAD, E., AZIMIRAD, M., NOCHI, Z., ROMANI, S., TAJBAKHSH, M., ROSTAMI NEJAD, M., HAGHIGHI, A., & ZALI, M.R.. (2012). SEQUENCE DIVERSITY IN TRNA GENE LOCUS A-L AMONG IRANIAN ISOLATES OF ENTAMOEBA DISPAR. IRANIAN JOURNAL OF PARASITOLOGY, 7(1), 97-103. SID. https://sid.ir/paper/304001/en

    Vancouver: Copy

    NAZEMALHOSSEINI MOJARAD E., AZIMIRAD M., NOCHI Z., ROMANI S., TAJBAKHSH M., ROSTAMI NEJAD M., HAGHIGHI A., ZALI M.R.. SEQUENCE DIVERSITY IN TRNA GENE LOCUS A-L AMONG IRANIAN ISOLATES OF ENTAMOEBA DISPAR. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2012;7(1):97-103. Available from: https://sid.ir/paper/304001/en

    IEEE: Copy

    E. NAZEMALHOSSEINI MOJARAD, M. AZIMIRAD, Z. NOCHI, S. ROMANI, M. TAJBAKHSH, M. ROSTAMI NEJAD, A. HAGHIGHI, and M.R. ZALI, “SEQUENCE DIVERSITY IN TRNA GENE LOCUS A-L AMONG IRANIAN ISOLATES OF ENTAMOEBA DISPAR,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 7, no. 1, pp. 97–103, 2012, [Online]. Available: https://sid.ir/paper/304001/en

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