مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

283
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

200
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

COMPARISON OF EIGHT CELL-FREE MEDIA FOR MAINTENANCE OF TOXOPLASMA GONDII TACHYZOITES (SHORT COMMUNICATION)

Pages

  104-109

Abstract

 Background: Toxoplasmosis is considered as one of the most common infectious diseases caused by the protozoan parasite TOXOPLASMA GONDII. TACHYZOITE is the main form of Toxoplasma and continuously is maintained in cell culture or injected into the mice peritoneal cavity. This study was designed to evaluate the survival rate of RH strain of T. gondii TACHYZOITEs in different cell free, nutrient and biological media at different temperatures.Methods: This experimental study was performed at the Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran, in 2010. One ml of each solution including hypotonic saline (0.3%), NORMAL SALINE (0.85%), RPMI-1640 (RPMI), RPMI with 10% fetal bovine serum (FBS), RPMI with 20% FBS, ovine hydatid cyst fluid, pasteurized milk of cow, and PHOSPHATE BUFFERED SALINE (PBS) along with 4×104 T. gondii TACHYZOITEs were added to plate wells and incubated in 4 oC, 22 oC, 37 oC, and 37 °C under 5% CO2. The survival rate and viability assessment of parasites were performed daily and the results were analyzed using Univariate tests.Result: TACHYZOITEs survival rate in PBS (4 °C) and NORMAL SALINE (4 oC) were considerably high, compared to other solutions in different conditions (P<0.001). The best temperature for Toxoplasma maintenance was 4 °C (P<0.001).Conclusion: This study introduces two available and economical solutions, PBS (4 oC) and NORMAL SALINE (4 oC) media, for maintenance of Toxoplasma TACHYZOITEs as appropriate choice media for a noticeable period of time (11 days) IN VITRO.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    KALANI, HAMED, DARYANI, AHMAD, SHARIF, MEHDI, AHMADPOUR, EHSAN, ALIZADEH, AHAD, NASROLAHEI, MOHTARAM, SARVI, SHAHABEDDIN, KALANI, FARZAD, & FARIDNIA, ROGHIYEH. (2016). COMPARISON OF EIGHT CELL-FREE MEDIA FOR MAINTENANCE OF TOXOPLASMA GONDII TACHYZOITES (SHORT COMMUNICATION). IRANIAN JOURNAL OF PARASITOLOGY, 11(1), 104-109. SID. https://sid.ir/paper/304069/en

    Vancouver: Copy

    KALANI HAMED, DARYANI AHMAD, SHARIF MEHDI, AHMADPOUR EHSAN, ALIZADEH AHAD, NASROLAHEI MOHTARAM, SARVI SHAHABEDDIN, KALANI FARZAD, FARIDNIA ROGHIYEH. COMPARISON OF EIGHT CELL-FREE MEDIA FOR MAINTENANCE OF TOXOPLASMA GONDII TACHYZOITES (SHORT COMMUNICATION). IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2016;11(1):104-109. Available from: https://sid.ir/paper/304069/en

    IEEE: Copy

    HAMED KALANI, AHMAD DARYANI, MEHDI SHARIF, EHSAN AHMADPOUR, AHAD ALIZADEH, MOHTARAM NASROLAHEI, SHAHABEDDIN SARVI, FARZAD KALANI, and ROGHIYEH FARIDNIA, “COMPARISON OF EIGHT CELL-FREE MEDIA FOR MAINTENANCE OF TOXOPLASMA GONDII TACHYZOITES (SHORT COMMUNICATION),” IRANIAN JOURNAL OF PARASITOLOGY, vol. 11, no. 1, pp. 104–109, 2016, [Online]. Available: https://sid.ir/paper/304069/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button