Induction of Apoptosis by Alcoholic Extract of Combination Verbascum thapsus and Ginger officinale on Iranian Isolate of Trichomonas vaginalis

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FAKHRIEH KASHAN ZOHREH; ARBABI MOHSEN; DELAVARI MAHDI; MOHEBALI MAHDI; HOOSHYAR HOSSEIN

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Journal: IRANIAN JOURNAL OF PARASITOLOGY Year:2018 | Volume:13 | Issue:1 Page(s): 72-78

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Title

Induction of Apoptosis by Alcoholic Extract of Combination Verbascum thapsus and Ginger officinale on Iranian Isolate of Trichomonas vaginalis

Author(s)

Keywords

Trichomonas vaginalis

Alcoholic extract

Verbascum thapsus

Ginger officinale

In vitro

Abstract

Background: The protozoan Trichomonas vaginalis is a sexually transmitted disease (STD). Metronidazole is a chosen drug for the treatment. This study evaluated the anti trichomonal activity of Alcoholic extracts of combination Verbascum thapsus and Ginger officinale. Methods: This experimental study was conducted in the Parasitology Laboratory, Kashan University of Medical Sciences, Kashan, Iran in 2015, on 23 women with suspected trichomoniasis referring to Kashan clinical centers. Medium TYIS-33 was used for culture of three T. vaginalis isolates. Different concentrations (25, 50, 100, 200, 400, 800 μ g/ml) of V. thapsus and G. officinale ethanol extract added to Trichomonas trophozoites in 48-well plates and metronidazole considered as positive control and the negative control was TYI-S33 containing Trichomonas trophozoites without any drug. In all of mentioned groups, trophozoites number counted 12, 24, 48 h after culture. Results were analyzed using ANOVA statistical test, to evaluate the toxicity of extract, measured by MTT assay. Induced apoptosis of T. vaginalis after treatment with different concentrations of extract was determined by Flow Cytometry. Results: IC50 of Alcoholic extract of combination V. thapsus and G. officinale and metronidazole after 24h was 73. 80 μ g/ml and 0. 0326 μ g/ml, respectively. The toxicity percentage of 25-800 μ g/ml concentrations of this combination were between 0. 2-1. 98. In different concentrations of extract (25, 50, 100, 200 and 400 μ g/ml) apoptosis percent after 48h was 18. 97 to 77. 19 and necrosis percent was calculated 1. 35, 3. 18, 3. 10, 1. 16 and 4. 09, respectively. Conclusion: Alcoholic extract of combination V. thapsus and G. officinale induces programmed death in T. vaginalis. Due to no toxicity on macrophages, it can be examined in vivo studies.

Cites

Antitrichomonal Effect of Methanolic Extract of Hyssopus Officinalis on Trichomonas vaginalis in Vitro

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APA: Copy

FAKHRIEH KASHAN, ZOHREH, ARBABI, MOHSEN, DELAVARI, MAHDI, MOHEBALI, MAHDI, & HOOSHYAR, HOSSEIN. (2018). Induction of Apoptosis by Alcoholic Extract of Combination Verbascum thapsus and Ginger officinale on Iranian Isolate of Trichomonas vaginalis. IRANIAN JOURNAL OF PARASITOLOGY, 13(1), 72-78. SID. https://sid.ir/paper/304124/en

Vancouver: Copy

FAKHRIEH KASHAN ZOHREH, ARBABI MOHSEN, DELAVARI MAHDI, MOHEBALI MAHDI, HOOSHYAR HOSSEIN. Induction of Apoptosis by Alcoholic Extract of Combination Verbascum thapsus and Ginger officinale on Iranian Isolate of Trichomonas vaginalis. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2018;13(1):72-78. Available from: https://sid.ir/paper/304124/en

IEEE: Copy

ZOHREH FAKHRIEH KASHAN, MOHSEN ARBABI, MAHDI DELAVARI, MAHDI MOHEBALI, and HOSSEIN HOOSHYAR, “Induction of Apoptosis by Alcoholic Extract of Combination Verbascum thapsus and Ginger officinale on Iranian Isolate of Trichomonas vaginalis,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 13, no. 1, pp. 72–78, 2018, [Online]. Available: https://sid.ir/paper/304124/en

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