Antitrichomonal Effect of Methanolic Extract of Hyssopus Officinalis on Trichomonas vaginalis in Vitro

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NOROUZI Roghayeh; ADNANI SADATI SEYED JAFAR; Yaghoubian Ramin; MIRZAEI FARZANEH; Fateh Ruhollah; Siadatpanah Abolghasem

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Journal: Qom University of Medical Sciences Journal Year:2021 | Volume:14 | Issue:12 Page(s): 14-21

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Title

Antitrichomonal Effect of Methanolic Extract of Hyssopus Officinalis on Trichomonas vaginalis in Vitro

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Keywords

Hyssopus officinalisQ1

Methanolic extractQ2

MetronidazoleQ1

Trichomonas vaginalisQ2

Culture mediumQ1

Abstract

Background and Objectives: Trichomonas vaginalis is a flagellate protozoan that is the most common sexually parasitic transmitted infection in the world. Metronidazole is currently used for the treatment of trichomoniasis, which has side effects. In recent years, the use of plants and natural compounds has been considered by researchers. This study was conducted to investigate the anti-trichomonal activity of Methanolic extract of Hyssopus officinalis on Trichomonas vaginalis in vitro. Methods: In this experimental study, the extract was prepared by Soxhlet method, and to evaluate the effect of Methanolic extract of Hyssopus officinalis on Trichomonas vaginalis parasites, the concentrations of 50, 100, 200, 400, 600, 800, 1, 000, 1, 200, and 1, 400 μ g/ml was prepared. Metronidazole and distilled water were considered as positive and negative controls, respectively. Afterward, 105 live parasites were added to all tubes, and all groups were kept at 37° C. Live parasites were counted at 24, 48, and 72 h intervals by Trypan Blue using a neobar slide and light microscope (Hemocytometer). Subsequently, the IC50 value for the above extract was calculated using SigmaPlot ™ software (version 13). It should be noted that all steps of the experiment were performed in triplicate and the results were considered as average. Results: The IC50 of Methanolic extract of Hyssopus officinalis on Trichomonas vaginalis parasite was calculated at 965. 8, 730. 4, and 542. 1 μ g/ml after 24, 48, and 72 h, respectively. The highest mortality rate (100%) was observed at a concentration of 1, 400 μ g/ml after 72 h of exposure. Conclusion: Methanolic extract of Hyssopus officinalis in different concentrations had an inhibitory effect on the growth of the Trichomonas vaginalis parasite. Further and more comprehensive studies are suggested to investigate the constituents of this plant and the lethal effect of the parasite in vivo conditions.

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APA: Copy

NOROUZI, Roghayeh, ADNANI SADATI, SEYED JAFAR, Yaghoubian, Ramin, MIRZAEI, FARZANEH, Fateh, Ruhollah, & Siadatpanah, Abolghasem. (2021). Antitrichomonal Effect of Methanolic Extract of Hyssopus Officinalis on Trichomonas vaginalis in Vitro. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, 14(12 ), 14-21. SID. https://sid.ir/paper/374550/en

Vancouver: Copy

NOROUZI Roghayeh, ADNANI SADATI SEYED JAFAR, Yaghoubian Ramin, MIRZAEI FARZANEH, Fateh Ruhollah, Siadatpanah Abolghasem. Antitrichomonal Effect of Methanolic Extract of Hyssopus Officinalis on Trichomonas vaginalis in Vitro. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL[Internet]. 2021;14(12 ):14-21. Available from: https://sid.ir/paper/374550/en

IEEE: Copy

Roghayeh NOROUZI, SEYED JAFAR ADNANI SADATI, Ramin Yaghoubian, FARZANEH MIRZAEI, Ruhollah Fateh, and Abolghasem Siadatpanah, “Antitrichomonal Effect of Methanolic Extract of Hyssopus Officinalis on Trichomonas vaginalis in Vitro,” QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, vol. 14, no. 12 , pp. 14–21, 2021, [Online]. Available: https://sid.ir/paper/374550/en

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