مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

233
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

132
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Synchronous Identification of Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp. in Stool Samples Using a Multiplex PCR Assay

Pages

  24-30

Abstract

 Background: Diarrheal disease annually causes 760000 deaths in children, and 1700 million new cases are reported each year worldwide. Among the parasites, Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp. are the most important infectious agents leading to diarrhea. Clinical presentations due to these parasites are more or less similar, and microscopy is not as much as sensitive for the detection. The aim of this study was to set up and evaluate a Multiplex PCR Assay for Synchronous Identification of Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp. in Stool Samples Methods: Samples were obtained from different sources such as culture media and patient stool samples. Primer pairs were designed using primer-BLAST, and for the extraction of DNA, the QIAamp DNA stool mini kit was used. The study was conducted in Tehran, Iran and completed in 2016. Results: The current Multiplex PCR assay for the detection of E. histolytica achieved sensitivity and specificity of 86. 36% (95% CI: 65. 09% to 97. 09) and 95. 74 % (95% CI: 85. 46% to 99. 48%), respectively. Sensitivity and specificity of the test for G. intestinalis was 90. 91% (95% CI: 70. 84% to 98. 88%) and 95. 74% (95%CI: 85. 46% to 99. 48%), respectively, and for the detection of Cryptosporidium, Multiplex PCR showed a sensitivity of 90. 91% (95% CI: 70. 84% to 98. 88%) and specificity of 95. 74% (95%CI: 85. 46% to 99. 48%). Conclusion: Multiplex PCR in this study showed admissible sensitivity and specificity for the detection of E. histolytica, G. intestinalis, and Cryptosporidium spp. in fecal samples.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    BAIRAMI, AMIR, REZAEI, SASAN, & REZAEIAN, MOSTAFA. (2018). Synchronous Identification of Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp. in Stool Samples Using a Multiplex PCR Assay. IRANIAN JOURNAL OF PARASITOLOGY, 13(1), 24-30. SID. https://sid.ir/paper/304132/en

    Vancouver: Copy

    BAIRAMI AMIR, REZAEI SASAN, REZAEIAN MOSTAFA. Synchronous Identification of Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp. in Stool Samples Using a Multiplex PCR Assay. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2018;13(1):24-30. Available from: https://sid.ir/paper/304132/en

    IEEE: Copy

    AMIR BAIRAMI, SASAN REZAEI, and MOSTAFA REZAEIAN, “Synchronous Identification of Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp. in Stool Samples Using a Multiplex PCR Assay,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 13, no. 1, pp. 24–30, 2018, [Online]. Available: https://sid.ir/paper/304132/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button