Detection of Potentially Diagnostic Leishmania Antigens with Western Blot Analysis of Sera from Patients with Cutaneous and Visceral Leishmaniases

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SEYYEDTABAEI SEYYED JAVAD; ROSTAMI ALI; HAGHIGHI ALI; MOHEBALI MEHDI; KAZEMI BAHRAM; FALLAHI SHIRZAD; SPOTIN ADEL

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Journal: IRANIAN JOURNAL OF PARASITOLOGY Year:2017 | Volume:12 | Issue:2 Page(s): 206-214

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Title

Detection of Potentially Diagnostic Leishmania Antigens with Western Blot Analysis of Sera from Patients with Cutaneous and Visceral Leishmaniases

Author(s)

Keywords

Visceral leishmaniasis

Cutaneous leishmaniasis

Diagnostic antigens

Western blot

IFAT

Abstract

Background: Visceral leishmaniasis (VL) and Cutaneous leishmaniasis (CL) are important public health problems in Iran. We aimed to evaluate the diagnostic potential of Western blot (WB) compared with indirect immunofluorescence test (IFAT) to serodiagnosis of leishmaniasis. Methods: This study was performed from 2010-2014 and participants were different parts of Iran. Serum samples were obtained from 43 patients with proven CL, 33 patients with proven VL, 39 patients with other parasitic diseases and 23 healthy individuals. Results: WB sensitivity for CL and VL was 100% and 91%, compared to IFA 4. 6% and 87. 8%, respectively. Sera from patients with CL and VL recognized numerous antigens with molecular weights ranging from 14 to 68 kDa and 12 to 94 kDa, respectively. The most sensitive antigens were 14 and 16 kDa for CL recognized by 100% of the sera from patients with proven CL and 12, 14 and 16 kDa for VL, recognized by 63. 6%, 100% and 63. 6% of the sera from patients with proven VL respectively. WB analysis is more sensitive than IFAT for the diagnosis of leishmaniasis particularly in cases of Cutaneous leishmaniasis. The 12, 14 and 16 kDa can be valuable diagnostic molecules for serodiagnosis of leishmaniasis because at least two immunogenic molecules were simultaneously detected by all patient sera, as well as produced antibodies against these antigens have no cross-reactivity with other control groups. Conclusion: WB could be useful for screening and serodiagnosis of CL and VL in epidemiologic studies in endemic areas.

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APA: Copy

SEYYEDTABAEI, SEYYED JAVAD, ROSTAMI, ALI, HAGHIGHI, ALI, MOHEBALI, MEHDI, KAZEMI, BAHRAM, FALLAHI, SHIRZAD, & SPOTIN, ADEL. (2017). Detection of Potentially Diagnostic Leishmania Antigens with Western Blot Analysis of Sera from Patients with Cutaneous and Visceral Leishmaniases. IRANIAN JOURNAL OF PARASITOLOGY, 12(2), 206-214. SID. https://sid.ir/paper/304185/en

Vancouver: Copy

SEYYEDTABAEI SEYYED JAVAD, ROSTAMI ALI, HAGHIGHI ALI, MOHEBALI MEHDI, KAZEMI BAHRAM, FALLAHI SHIRZAD, SPOTIN ADEL. Detection of Potentially Diagnostic Leishmania Antigens with Western Blot Analysis of Sera from Patients with Cutaneous and Visceral Leishmaniases. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2017;12(2):206-214. Available from: https://sid.ir/paper/304185/en

IEEE: Copy

SEYYED JAVAD SEYYEDTABAEI, ALI ROSTAMI, ALI HAGHIGHI, MEHDI MOHEBALI, BAHRAM KAZEMI, SHIRZAD FALLAHI, and ADEL SPOTIN, “Detection of Potentially Diagnostic Leishmania Antigens with Western Blot Analysis of Sera from Patients with Cutaneous and Visceral Leishmaniases,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 12, no. 2, pp. 206–214, 2017, [Online]. Available: https://sid.ir/paper/304185/en

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