Evaluation of Microsatellites Markers to Discriminate Four Main Taeniid Tapeworms of Dogs (Short Communication)

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SHAMSADDINI Saeedeh; MOHAMMADI MOHAMMAD ALI; MIRBADIE Seyed Reza; NASIBI Saeed; ROSTAMI SIMA; DEHGHANI MANSOUREH; FASIHI HARANDI MAJID

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Journal: IRANIAN JOURNAL OF PARASITOLOGY Year:2017 | Volume:12 | Issue:2 Page(s): 292-297

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Title

Evaluation of Microsatellites Markers to Discriminate Four Main Taeniid Tapeworms of Dogs (Short Communication)

Author(s)

Keywords

Echinococcus

Taenia

Microsatellite markers

Transmission

EmsB

Abstract

Background: Echinococcus granulosus, Taenia multiceps, Taenia ovis and Taenia hydatigena are among the most prevalent taeniid species of dogs. These tapeworms infect ruminant and humans as intermediate hosts and domestic/wild carnivores as the definitive hosts. Molecular tools using hypervariable microsatellite regions might provide more information about parasite variation. Highly variable and specific tools are needed for Transmission tracking studies of canine echinococcosis as an essential element for implementation of hydatid control programs. Suitable Microsatellite markers used so far are EmsJ, EmsK, EmsB, EMms1, Egmsca1, Egmsga1, U1 snRNA. The purpose of the present study was to determine the microsatellite variability of EmsB as well as six other microsatellites in major taeniid species infecting dogs in Iran. Methods: Twenty isolates of each of the four Taeniidae tapeworms were collected from sheep during routine veterinary inspection in Tehran, Alborz and Kerman provinces from October 2010 to May 2011. After DNA extraction, PCR was set up with optimum conditions using specific primers for each individual microsatellite marker. All the PCR products were evaluated by agarose gel electrophoresis. We used SDSPAGE for evaluating patterns of PCR products in the tapeworms. Results: E. granulosus as well as Taenia species could be differentiated based on EmsB microsatellite patterns. The electrophoresis patterns of two taeniid genera were readily distinguishable. EmsB could be specifically used in epidemiological studies of canine echinococcosis. Conclusion: Different patterns of EmsB proved this microsatellite marker as a reliable tool for epidemiological studies on canine echinococcosis.

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APA: Copy

SHAMSADDINI, Saeedeh, MOHAMMADI, MOHAMMAD ALI, MIRBADIE, Seyed Reza, NASIBI, Saeed, ROSTAMI, SIMA, DEHGHANI, MANSOUREH, & FASIHI HARANDI, MAJID. (2017). Evaluation of Microsatellites Markers to Discriminate Four Main Taeniid Tapeworms of Dogs (Short Communication). IRANIAN JOURNAL OF PARASITOLOGY, 12(2), 292-297. SID. https://sid.ir/paper/304191/en

Vancouver: Copy

SHAMSADDINI Saeedeh, MOHAMMADI MOHAMMAD ALI, MIRBADIE Seyed Reza, NASIBI Saeed, ROSTAMI SIMA, DEHGHANI MANSOUREH, FASIHI HARANDI MAJID. Evaluation of Microsatellites Markers to Discriminate Four Main Taeniid Tapeworms of Dogs (Short Communication). IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2017;12(2):292-297. Available from: https://sid.ir/paper/304191/en

IEEE: Copy

Saeedeh SHAMSADDINI, MOHAMMAD ALI MOHAMMADI, Seyed Reza MIRBADIE, Saeed NASIBI, SIMA ROSTAMI, MANSOUREH DEHGHANI, and MAJID FASIHI HARANDI, “Evaluation of Microsatellites Markers to Discriminate Four Main Taeniid Tapeworms of Dogs (Short Communication),” IRANIAN JOURNAL OF PARASITOLOGY, vol. 12, no. 2, pp. 292–297, 2017, [Online]. Available: https://sid.ir/paper/304191/en

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