مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

245
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

146
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

1

Information Journal Paper

Title

Comparison of Three Different DNA Extraction Methods for Linguatula serrata as a Food Born Pathogen

Pages

  236-242

Abstract

 Background: One of the most important items in molecular characterization of food-borne pathogens is high quality genomic DNA. In this study, we investigated three protocols and compared their simplicity, duration and costs for extracting genomic DNA from Linguatula serrata. Methods: The larvae were collected from the sheep’ s visceral organs from the Yazd Slaughterhouse during May 2013. DNA extraction was done in three different methods, including commercial DNA extraction kit, Phenol Chloroform Isoamylalcohol (PCI), and salting out. Extracted DNA in each method was assessed for quantity and quality using spectrophotometery and agarose gel electrophoresis, respectively. Results: The less duration was regarding to commercial DNA extraction kit and then salting out protocol. The cost benefit one was salting out and then PCI method. The best quantity was regarding to PCI with 72. 20± 29. 20 ng/μ l, and purity of OD260/OD280 in 1. 76± 0. 947. Agarose gel electrophoresis for assessing the quality found all the same. Conclusion: Salting out is introduced as the best method for DNA extraction from L. seratta as a food-borne pathogen with the least costand appropriate purity. Although, the best purity was regarding to PCI but PCI is not safe as salting out. In addition, the duration of salting out was less than PCI. The least duration was seen in commercial DNA extraction kit, but it is expensive and therefore is not recommended for developing countries where consumption of offal is common.

Cites

References

  • No record.

    • Cite

    APA: Copy

    ESLAMI, GILDA, KHALATBARI LIMAKI, Sepideh, EHRAMPOUSH, MOHAMMAD HASAN, GHOLAMREZAEI, MOSTAFA, HAJIMOHAMMADI, BAHADOR, & ORYAN, AHMAD. (2017). Comparison of Three Different DNA Extraction Methods for Linguatula serrata as a Food Born Pathogen. IRANIAN JOURNAL OF PARASITOLOGY, 12(2), 236-242. SID. https://sid.ir/paper/304193/en

    Vancouver: Copy

    ESLAMI GILDA, KHALATBARI LIMAKI Sepideh, EHRAMPOUSH MOHAMMAD HASAN, GHOLAMREZAEI MOSTAFA, HAJIMOHAMMADI BAHADOR, ORYAN AHMAD. Comparison of Three Different DNA Extraction Methods for Linguatula serrata as a Food Born Pathogen. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2017;12(2):236-242. Available from: https://sid.ir/paper/304193/en

    IEEE: Copy

    GILDA ESLAMI, Sepideh KHALATBARI LIMAKI, MOHAMMAD HASAN EHRAMPOUSH, MOSTAFA GHOLAMREZAEI, BAHADOR HAJIMOHAMMADI, and AHMAD ORYAN, “Comparison of Three Different DNA Extraction Methods for Linguatula serrata as a Food Born Pathogen,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 12, no. 2, pp. 236–242, 2017, [Online]. Available: https://sid.ir/paper/304193/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button