Leishmania major: Genetic Profiles of the Parasites Isolated from Chabahar, Southeastern Iran by PPIP-PCR

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SHARIFI RAD Mehdi; DABIRZADEH MANSOUR; SHARIFI IRAJ; BABAEI ZAHRA

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Journal Paper

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Journal: IRANIAN JOURNAL OF PARASITOLOGY Year:2016 | Volume:11 | Issue:3 Page(s): 290-295

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Title

Leishmania major: Genetic Profiles of the Parasites Isolated from Chabahar, Southeastern Iran by PPIP-PCR

Author(s)

SHARIFI RAD Mehdi | DABIRZADEH MANSOUR | SHARIFI IRAJ | BABAEI ZAHRA | Issue Writer Certificate

Keywords

PPIP-PCR

Leishmania major

Genetic polymorphism

Iran

Abstract

Background: Leishmaniasis is important vector-borne parasitic disease worldwide, caused by the genus Leishmania. The objective of the current study was to identify Genetic polymorphism in L. major, one of the species causing cutaneous leishmaniasis (CL), isolated from southeastern Iran, using Permissively Primed Intergenic Polymorphic-Polymerase Chain Reaction (PPIP-PCR) method. Methods: Overall, 340 patients with suspected CL were examined. They referred to the Central Laboratory in Chabahar, Iran during Apr 2013 to Feb 2014. Microscopic examination of Giemsa-stained slides from lesions as well as aspirates cultured in Novy-Mac Neal-Nicolle (NNN) Media was employed in order to diagnose CL in these patients. Our analyses detected 86 suspected subjects as having CL from which 35 isolates were cultured successfully. PPIP-PCR method was performed on extracted genomic DNA from selected isolates in order to determine the Genetic polymorphism among L. major isolates. Results: The electrophoresis patterns demonstrated two genetic profiles including A or A1 patterns between all samples tested. Frequency of A and A1 sub-types were 33 (94. 3%) and two (5. 7%), respectively. Conclusion: Both host and parasite factors may contribute to the clinical profile of human leishmaniasis in the endemic foci of the disease. Here we showed that genetic variations pertaining to the Leishmania parasites might determine, in part, the clinical outcomes of human leishmaniasis.

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APA: Copy

SHARIFI RAD, Mehdi, DABIRZADEH, MANSOUR, SHARIFI, IRAJ, & BABAEI, ZAHRA. (2016). Leishmania major: Genetic Profiles of the Parasites Isolated from Chabahar, Southeastern Iran by PPIP-PCR. IRANIAN JOURNAL OF PARASITOLOGY, 11(3), 290-295. SID. https://sid.ir/paper/304209/en

Vancouver: Copy

SHARIFI RAD Mehdi, DABIRZADEH MANSOUR, SHARIFI IRAJ, BABAEI ZAHRA. Leishmania major: Genetic Profiles of the Parasites Isolated from Chabahar, Southeastern Iran by PPIP-PCR. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2016;11(3):290-295. Available from: https://sid.ir/paper/304209/en

IEEE: Copy

Mehdi SHARIFI RAD, MANSOUR DABIRZADEH, IRAJ SHARIFI, and ZAHRA BABAEI, “Leishmania major: Genetic Profiles of the Parasites Isolated from Chabahar, Southeastern Iran by PPIP-PCR,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 11, no. 3, pp. 290–295, 2016, [Online]. Available: https://sid.ir/paper/304209/en

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