Trypanosoma cruzi: Evaluation of PCR as a Laboratory Tool to Follow up the Evolution of Parasite Load (Short Communication)

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FERRAZ Fabiana Nabarro; ALEIXO Denise Lessa; GRUENDLING Ana Paula; GOMES Monica Lucia; de Ornelas TOLEDO Max Jean; DE ARAuJO Silvana Marques

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Journal: IRANIAN JOURNAL OF PARASITOLOGY Year:2016 | Volume:11 | Issue:3 Page(s): 389-395

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Title

Trypanosoma cruzi: Evaluation of PCR as a Laboratory Tool to Follow up the Evolution of Parasite Load (Short Communication)

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Keywords

Trypanosoma cruzi

Parasite load

PCR

kDNA fragments

Abstract

Background: The study evaluated qualitative PCR, primers 121-122 as a tool to follow up evolution Parasite load of Trypanosoma cruzi. Methods: The study was conducted at the State University of Maringa, in 2015. Step 1, dilutions 1/10 were performed from T. cruzi-Y strain to obtain preparations of 50, 000-0. 05 parasites/mL from which DNA were extracted, quantified, and amplified. Step 2, the extracted DNA in the dilutions 5-0. 05 parasites/mL was re-diluted 1/10, 1/100, 1/1000, quantified, and amplified. Polyacrylamide gels were photographed and thicknesses of the 330 bp kDNA fragments were measured. Results: Step 1, in the dilutions 50, 000-50 parasites/mL kDNA fragments had same thickness and, dilutions 5-0. 05 parasites/mL showed progressive decrease in thicknesses and staining intensity of the 330 bp fragments. Step 2, demonstrated that dilutions of five (re-dilutions 1/10 and 1/100) and 0. 5 (1/10) parasites/mL produced similar thicknesses of the 330 bp fragments obtained in Step 1. However, very dilute DNA samples make difficult to reproduce the fragments thicknesses. Conclusion: PCR, despite its limitations, was able to detect progressive decrease in thicknesses/staining intensity of kDNA fragments in the dilutions 5-0. 05 parasites/mL. Hence, has the potential to be used to follow-up evolution of Parasite load, not by quantifying the number of parasites, but by dynamic evolution of the fragments thicknesses during etiological treatment.

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APA: Copy

FERRAZ, Fabiana Nabarro, ALEIXO, Denise Lessa, GRUENDLING, Ana Paula, GOMES, Monica Lucia, de Ornelas TOLEDO, Max Jean, & DE ARAuJO, Silvana Marques. (2016). Trypanosoma cruzi: Evaluation of PCR as a Laboratory Tool to Follow up the Evolution of Parasite Load (Short Communication). IRANIAN JOURNAL OF PARASITOLOGY, 11(3), 389-395. SID. https://sid.ir/paper/304213/en

Vancouver: Copy

FERRAZ Fabiana Nabarro, ALEIXO Denise Lessa, GRUENDLING Ana Paula, GOMES Monica Lucia, de Ornelas TOLEDO Max Jean, DE ARAuJO Silvana Marques. Trypanosoma cruzi: Evaluation of PCR as a Laboratory Tool to Follow up the Evolution of Parasite Load (Short Communication). IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2016;11(3):389-395. Available from: https://sid.ir/paper/304213/en

IEEE: Copy

Fabiana Nabarro FERRAZ, Denise Lessa ALEIXO, Ana Paula GRUENDLING, Monica Lucia GOMES, Max Jean de Ornelas TOLEDO, and Silvana Marques DE ARAuJO, “Trypanosoma cruzi: Evaluation of PCR as a Laboratory Tool to Follow up the Evolution of Parasite Load (Short Communication),” IRANIAN JOURNAL OF PARASITOLOGY, vol. 11, no. 3, pp. 389–395, 2016, [Online]. Available: https://sid.ir/paper/304213/en

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