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Information Journal Paper

Title

Sequence Variation in Rhoptry Neck Protein 10 Gene among Toxoplasma gondii Isolates from Different Hosts and Geographical Locations

Pages

  332-338

Keywords

Rhoptry neck protein 10 (TgRON10) 

Abstract

 Background: Toxoplasma gondii, as a eukaryotic parasite of the phylum Apicomplexa, can infect almost all the warm-blooded animals and humans, causing Toxoplasmosis. Rhoptry neck proteins (RONs) play a key role in the invasion process of T. gondii and are potential vaccine candidate molecules against Toxoplasmosis. Methods: The present study examined Sequence variation in the rhoptry neck protein 10 (TgRON10) gene among 10 T. gondii isolates from different hosts and geographical locations from Lanzhou province during 2014, and compared with the corresponding sequences of strains ME49 and VEG obtained from the ToxoDB database, using polymerase chain reaction (PCR) amplification, sequence analysis, and phylogenetic reconstruction by Bayesian inference (BI) and maximum parsimony (MP). Results: Analysis of all the 12 TgRON10 genomic and cDNA sequences revealed 7 exons and 6 introns in the TgRON10 gDNA. The complete genomic sequence of the TgRON10 gene ranged from 4759 bp to 4763 bp, and Sequence variation was 0-0. 6% among the 12 T. gondii isolates, indicating a low Sequence variation in TgRON10 gene. Phylogenetic analysis of TgRON10 sequences showed that the cluster of the 12 T. gondii isolates was not completely consistent with their respective genotypes. Conclusion: TgRON10 gene is not a suitable genetic marker for the differentiation of T. gondii isolates from different hosts and geographical locations, but may represent a potential vaccine candidate against Toxoplasmosis, worth further studies.

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    • Cite

    APA: Copy

    ZHOU, Donghui, CHEN, Jia, & SUN, Xiaolin. (2017). Sequence Variation in Rhoptry Neck Protein 10 Gene among Toxoplasma gondii Isolates from Different Hosts and Geographical Locations. IRANIAN JOURNAL OF PARASITOLOGY, 12(3), 332-338. SID. https://sid.ir/paper/304218/en

    Vancouver: Copy

    ZHOU Donghui, CHEN Jia, SUN Xiaolin. Sequence Variation in Rhoptry Neck Protein 10 Gene among Toxoplasma gondii Isolates from Different Hosts and Geographical Locations. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2017;12(3):332-338. Available from: https://sid.ir/paper/304218/en

    IEEE: Copy

    Donghui ZHOU, Jia CHEN, and Xiaolin SUN, “Sequence Variation in Rhoptry Neck Protein 10 Gene among Toxoplasma gondii Isolates from Different Hosts and Geographical Locations,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 12, no. 3, pp. 332–338, 2017, [Online]. Available: https://sid.ir/paper/304218/en

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