Molecular Detection of Dirofilaria immitis Specific Gene from Infected Dog Blood Sample Using Polymerase Chain Reaction

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OH In Young; KIM Kyung Tae; SUNG Ho Joong

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Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF PARASITOLOGY Year:2017 | Volume:12 | Issue:3 Page(s): 433-440

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Title

Molecular Detection of Dirofilaria immitis Specific Gene from Infected Dog Blood Sample Using Polymerase Chain Reaction

Author(s)

OH In Young | KIM Kyung Tae | SUNG Ho Joong | Issue Writer Certificate

Keywords

Dirofilaria immitis

COI

Molecular diagnosis

PCR

Abstract

Background: Dirofilaria immitis, a filarial nematode, is the most important parasiteaffecting dogs, causing cardiopulmonary dirofilariasis. Current diagnostic tools for detecting D. immitis include morphological assays, antigen detection, and X-ray. Herein, we developed a method for the molecular detection of D. immitis in blood using polymerase chain reaction (PCR). Methods: The study was conducted at Eulji University, Republic of Korea in 2016. To detect D. immitis-specific gene regions, we aligned the cytochrome c oxidase subunit I (COI) genes of seven filarial nematodes and designed primers targeting the unique region. We used dog glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-targeted primers as the internal control. We conducted PCR-amplified genomic DNA from canine blood samples. The products were confirmed by sequencing. Results: Gene alignment revealed a D. immitis COI-specific gene region, and the activity of designed primers was confirmed by PCR and sequencing. Plasmid DNA made from the PCR products was a positive control. The limit of detection for our method was 50 copies. The D. immitis COI and dog GAPDH genes could be discriminated from blood samples simultaneously. Conclusion: This study provides a method for highly specific and sensitive Molecular diagnosis of D. immitis used as a diagnostic and therapeutic tool from the early stage of infection.

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APA: Copy

OH, In Young, KIM, Kyung Tae, & SUNG, Ho Joong. (2017). Molecular Detection of Dirofilaria immitis Specific Gene from Infected Dog Blood Sample Using Polymerase Chain Reaction. IRANIAN JOURNAL OF PARASITOLOGY, 12(3), 433-440. SID. https://sid.ir/paper/304230/en

Vancouver: Copy

OH In Young, KIM Kyung Tae, SUNG Ho Joong. Molecular Detection of Dirofilaria immitis Specific Gene from Infected Dog Blood Sample Using Polymerase Chain Reaction. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2017;12(3):433-440. Available from: https://sid.ir/paper/304230/en

IEEE: Copy

In Young OH, Kyung Tae KIM, and Ho Joong SUNG, “Molecular Detection of Dirofilaria immitis Specific Gene from Infected Dog Blood Sample Using Polymerase Chain Reaction,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 12, no. 3, pp. 433–440, 2017, [Online]. Available: https://sid.ir/paper/304230/en

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