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Information Journal Paper

Title

EFFECT OF FIBROBLAST CO-CULTURE ON IN VITRO MATURATION AND FERTILIZATION OF MOUSE PREANTRAL FOLLICLES

Pages

  1-8

Abstract

 Background: The aim of this study was to evaluate FIBROBLAST CO-CULTURE on in vitro maturation and fertilization of prepubertal mouse PREANTRAL FOLLICLEs.Materials and Methods: The ovaries of 12-14 day old mice were dissected and 120-150 μm intact PREANTRAL FOLLICLEs with one or two layers of granulosa cells, and round oocytes were cultured individually inα-minimal essential medium (α-MEM) supplemented with 5% fetal bovine serum (FBS), 100 mIU/ml recombinant FOLLICLE stimulating hormone, 1% insulin, transferrin, selenium mix, 100μg/ml penicillin and 50 μg/ml streptomycin as base medium for 12 days. A total number of 226 follicules were cultured under two conditions: i) base medium as control group (n=113); ii) base medium CO-CULTUREd with mouse embryonic FIBROBLAST (MEF) (n=113). Follicular diameters, alone, in addition to other factors were analyzed by student’s t-test and chi-square test, respectively.Results: The CO-CULTURE group showed significant differences (p<0.05) in growth rate (days 4, 6 and 8 of the culture period) and survival rate. However, there was no significant difference in antrum formation, ovulation rate and embryonic development of released oocytes. There were significant differences (p<0.05) in the estradiol and progesterone secretion at all days between the CO-CULTURE and control groups.Conclusion: FIBROBLAST CO-CULTURE increased survival rate and steroid production of PREANTRAL FOLLICLEs by promoting granulosa cell proliferation.

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    APA: Copy

    HEIDARI, MAHMOUD, KARIMPOUR MALEKSHAH, ABBASALI, PARIVAR, KAZEM, KHANBABAEI, RAMEZAN, & RAFIEI, ALIREZA. (2011). EFFECT OF FIBROBLAST CO-CULTURE ON IN VITRO MATURATION AND FERTILIZATION OF MOUSE PREANTRAL FOLLICLES. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 5(1), 1-8. SID. https://sid.ir/paper/304733/en

    Vancouver: Copy

    HEIDARI MAHMOUD, KARIMPOUR MALEKSHAH ABBASALI, PARIVAR KAZEM, KHANBABAEI RAMEZAN, RAFIEI ALIREZA. EFFECT OF FIBROBLAST CO-CULTURE ON IN VITRO MATURATION AND FERTILIZATION OF MOUSE PREANTRAL FOLLICLES. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY[Internet]. 2011;5(1):1-8. Available from: https://sid.ir/paper/304733/en

    IEEE: Copy

    MAHMOUD HEIDARI, ABBASALI KARIMPOUR MALEKSHAH, KAZEM PARIVAR, RAMEZAN KHANBABAEI, and ALIREZA RAFIEI, “EFFECT OF FIBROBLAST CO-CULTURE ON IN VITRO MATURATION AND FERTILIZATION OF MOUSE PREANTRAL FOLLICLES,” INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, vol. 5, no. 1, pp. 1–8, 2011, [Online]. Available: https://sid.ir/paper/304733/en

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