GENETICS: PHOSPHOLIPASE C ZETA: AS AN INDEX TO ASSESS FERTILIZATION POTENTIAL OF A SEMEN SAMPLE

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AGHAJANPOUR S.; GHAEDI K.; SALAMIAN A.; TAVALAEE M.; DEEMEH M.R.; NASR ESFAHANI M.H.

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Journal Paper

Paper Information

Journal: International Journal of Fertility & Sterility Year:2011 | Volume:5 | Issue:SUPPLEMENT 1 Page(s): 74-74

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Title

GENETICS: PHOSPHOLIPASE C ZETA: AS AN INDEX TO ASSESS FERTILIZATION POTENTIAL OF A SEMEN SAMPLE

Author(s)

Keywords

FAILED FERTILIZATION

PLCϚ

CROSIN ACTIVITY

PROTAMINE

ICSI

Abstract

Background: FAILED FERTILIZATION post ICSI has been mainly attributed to the sperm’s inability to induce oocyte activation. Phospholipase C Zeta (PLCz) is considered to be a primary factor for the induction of oocyte activation. The aim of this study was to quantitatively assess the expression of PLCz in globozoospermic or individuals with previous low or FAILED FERTILIZATION in comparison to fertile or individuals with high fertilization potential.Materials and Methods: Real-time PCR was carried out to evaluate relative expression of PLCz mRNA.Results: The expression of PLCz was significantly lower in globozoospermic men or individuals with previous low or FAILED FERTILIZATION in comparison to fertile individuals or those with high fertilization. Significant correlation was observed between relative expression of PLCz and fertilization rate.Conclusion: In this study, for the first time, we have shown that assessment of relative expression of PLCz appears to provide a good index for quantitative assessment of the potential for a semen sample to induce oocyte activation.

Cites

Effects of assisted oocyte activation with calcium-ionophore and strontium chloride on in vitro ICSI outcomes

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APA: Copy

AGHAJANPOUR, S., GHAEDI, K., SALAMIAN, A., TAVALAEE, M., DEEMEH, M.R., & NASR ESFAHANI, M.H.. (2011). GENETICS: PHOSPHOLIPASE C ZETA: AS AN INDEX TO ASSESS FERTILIZATION POTENTIAL OF A SEMEN SAMPLE. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 5(SUPPLEMENT 1), 74-74. SID. https://sid.ir/paper/304883/en

Vancouver: Copy

AGHAJANPOUR S., GHAEDI K., SALAMIAN A., TAVALAEE M., DEEMEH M.R., NASR ESFAHANI M.H.. GENETICS: PHOSPHOLIPASE C ZETA: AS AN INDEX TO ASSESS FERTILIZATION POTENTIAL OF A SEMEN SAMPLE. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY[Internet]. 2011;5(SUPPLEMENT 1):74-74. Available from: https://sid.ir/paper/304883/en

IEEE: Copy

S. AGHAJANPOUR, K. GHAEDI, A. SALAMIAN, M. TAVALAEE, M.R. DEEMEH, and M.H. NASR ESFAHANI, “GENETICS: PHOSPHOLIPASE C ZETA: AS AN INDEX TO ASSESS FERTILIZATION POTENTIAL OF A SEMEN SAMPLE,” INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, vol. 5, no. SUPPLEMENT 1, pp. 74–74, 2011, [Online]. Available: https://sid.ir/paper/304883/en

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