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Information Journal Paper

Title

A LOW-COST EFFICIENT MULTIPLEX P CR FOR PRENATAL SEX DETERMINATION IN BOVINE FETUS U SING FREE FETAL DNA IN MATERNAL PLASMA

Pages

  45-50

Abstract

 Background: In order to establish a reliable non-invasive method for SEX DETERMINATION in a bovine fetus in a routine setting, the possibility of identifying specific sequence in the fetal X and Y-chromosomes has been evaluated in MATERNAL PLASMA using conventional multiplex polymerase chain reaction (PCR) analysis. The aim of this study was to provide a rapid and reliable method for sexing bovine fetuses.Materials and Methods: In this experimental study, peripheral blood samples were taken from 38 pregnant heifers with 8 to 38 weeks of gestation. DNA template was extracted by phenol-chloroform method from 350 ml MATERNAL PLASMA. Two primer pairs for bovine amelogenin gene (bAML) and BC1.2 were used to amplify fragments from X and Y chromosomes. A MULTIPLEX PCR reaction has been optimized for amplification of 467 bp and 341 bp fragments from X and Y bAML gene and a 190 bp fragment from BC1.2 related to Y chromosome.Results: The 467 bp fragment was observed in all 38 samples. Both 341 and 190 bp fragments were detected only in 24 plasma samples from male calves. The sensitivity and specificity of test were 100% with no false negative or false positive results.Conclusion: The results showed that phenol-chloroform method is a simple and suitable method for isolation of fetal DNA in MATERNAL PLASMA. The MULTIPLEX PCR method is an available non-invasive approach which is cost efficient and reliable for sexing bovine fetuses.

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    APA: Copy

    DAVOUDI, ARASH, SEIGHALANI, RAMIN, ALEYASIN, SEYED AHMAD, TARANG, ALIREZA, SALEHI, ABDOLREZA, & TAHMORESSI, FARIDEH. (2012). A LOW-COST EFFICIENT MULTIPLEX P CR FOR PRENATAL SEX DETERMINATION IN BOVINE FETUS U SING FREE FETAL DNA IN MATERNAL PLASMA. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 6(1), 45-50. SID. https://sid.ir/paper/304993/en

    Vancouver: Copy

    DAVOUDI ARASH, SEIGHALANI RAMIN, ALEYASIN SEYED AHMAD, TARANG ALIREZA, SALEHI ABDOLREZA, TAHMORESSI FARIDEH. A LOW-COST EFFICIENT MULTIPLEX P CR FOR PRENATAL SEX DETERMINATION IN BOVINE FETUS U SING FREE FETAL DNA IN MATERNAL PLASMA. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY[Internet]. 2012;6(1):45-50. Available from: https://sid.ir/paper/304993/en

    IEEE: Copy

    ARASH DAVOUDI, RAMIN SEIGHALANI, SEYED AHMAD ALEYASIN, ALIREZA TARANG, ABDOLREZA SALEHI, and FARIDEH TAHMORESSI, “A LOW-COST EFFICIENT MULTIPLEX P CR FOR PRENATAL SEX DETERMINATION IN BOVINE FETUS U SING FREE FETAL DNA IN MATERNAL PLASMA,” INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, vol. 6, no. 1, pp. 45–50, 2012, [Online]. Available: https://sid.ir/paper/304993/en

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