COMPAISON OF SWIM UP AND HISTOPREP® CONTINUOUS GRADIENT FOR ISOLATION OF MOTILE EPIDIDYMAL SPERM IN RAM

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SHOKROLLAHI E.; BARATI F.; GOORANINEJAD S.

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Journal Paper

Paper Information

Journal: International Journal of Fertility & Sterility Year:2012 | Volume:6 | Issue:SUPPLEMENT 1 Page(s): 99-99

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Title

COMPAISON OF SWIM UP AND HISTOPREP® CONTINUOUS GRADIENT FOR ISOLATION OF MOTILE EPIDIDYMAL SPERM IN RAM

Author(s)

SHOKROLLAHI E. | BARATI F. | GOORANINEJAD S.

Keywords

EPIDIDYMAL SPERM

SWIM UP

HISTOPREP

RAM

Abstract

Background: Isolation of motile sperm is essential for routine IVF programs.One of most important source of sperm for IVF is isolation of EPIDIDYMAL SPERM (ESP).The problem regarding use of ESP is somatic cells which are recovered through sperm collection. The SWIM UP and the swim down procedures are use to isolate motile sperm. The aim of the present study was to compare SWIM UP and HISTOPREP®continuous gradient (HP) for isolation of RAM ESP.Materials and Methods: RAM testes epididymes were collected from local slaughterhouse and transported to the Lab. Basic medium for separation of sperms from epididymis was caffeine treated BO solution. HISTOPREP was provided from the company. After incubating of cauda epididymis in the basic medium for 15 minutes, primary sperm parameters including sperm motility and concentration were recorded. Two equal volumes of sperm solution were use in SWIM UP solution and on HISTOPREP gradient. We followed the routine SWIM UP procedure by incubation of 800 ml of BO-caff in CO2 incubator for 30-45 minutes. For HISTOPREP, we followed the company protocol for isolation of human lymphocytes.After sperm recovery in two procedures, the secondary sperm parameters were recorded. To find the effects of the procedure on sperm parameters, proportion of secondary to primary sperm parameters, for each method, was calculated.Results: The results showed that proportion of motile sperms were 1.094±0.04 and 1.19±0.06 in HISTOPREP and SWIM UP respectively (p>0.05). The proportion of secondary to primary sperm count for two procedure was similar (0.27±0.05 and 0.25±0.07 for HISTOPREP and SWIM UP, respectively).Conclusion: The results showed that HISTOPREP and SWIM UP have similar effects on motile sperm isolation for RAM ESP.

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APA: Copy

SHOKROLLAHI, E., BARATI, F., & GOORANINEJAD, S.. (2012). COMPAISON OF SWIM UP AND HISTOPREP^® CONTINUOUS GRADIENT FOR ISOLATION OF MOTILE EPIDIDYMAL SPERM IN RAM. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 6(SUPPLEMENT 1), 99-99. SID. https://sid.ir/paper/305311/en

Vancouver: Copy

SHOKROLLAHI E., BARATI F., GOORANINEJAD S.. COMPAISON OF SWIM UP AND HISTOPREP^® CONTINUOUS GRADIENT FOR ISOLATION OF MOTILE EPIDIDYMAL SPERM IN RAM. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY[Internet]. 2012;6(SUPPLEMENT 1):99-99. Available from: https://sid.ir/paper/305311/en

IEEE: Copy

E. SHOKROLLAHI, F. BARATI, and S. GOORANINEJAD, “COMPAISON OF SWIM UP AND HISTOPREP^® CONTINUOUS GRADIENT FOR ISOLATION OF MOTILE EPIDIDYMAL SPERM IN RAM,” INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, vol. 6, no. SUPPLEMENT 1, pp. 99–99, 2012, [Online]. Available: https://sid.ir/paper/305311/en

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