EXPRESSION LEVELS OF PPARΓ AND CYP-19 IN POLYCYSTIC OVARIAN SYNDROME PRIMARY GRANULOSA CELLS: INFLUENCE OF ω-3 FATTY ACID

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ZAREEI MINA; SHAHNAZI VAHIDEH; FAYEZI SHABNAM; DARABI MARYAM; MEHRZAD SADAGHIANI MAHZAD; DARABI MASOUD; KHANI SAJJAD; NOURI MOHAMMAD

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Journal: International Journal of Fertility & Sterility Year:2015 | Volume:9 | Issue:2 Page(s): 197-204

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Title

EXPRESSION LEVELS OF PPARΓ AND CYP-19 IN POLYCYSTIC OVARIAN SYNDROME PRIMARY GRANULOSA CELLS: INFLUENCE OF ω-3 FATTY ACID

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Keywords

EICOSAPENTAENOIC ACID

PPAR GAMMA

AROMATASE

GRANULOSA CELLS

POLYCYSTIC OVARY SYNDROME

Abstract

Background: The omega-3 fatty acid (w-3 fatty acid) such as EICOSAPENTAENOIC ACID (EPA) is currently used in the clinic as a nutritional supplement in the treatment of polycystic ovarian syndrome (PCOS). The present study was designed to investigate the effect of EPA on the expression levels of peroxisome proliferator-activated receptor gamma (PPARg) and cytochrome P450 AROMATASE (encoded by the CYP-19) in primary cultured GRANULOSA CELLS (GC) from patients undergoing in vitro fertilization (IVF), and also to compare these effects with those in GC of PCOS patients.Materials and Methods: In this experimental study, human GC were isolated, primary cultured in vitro, exposed to a range of concentrations of the EPA and investigated with respect to gene expression levels of PPARg and CYP-19 using real time-polymerase chain reaction (PCR). The participants (n=30) were the patients admitted to the IVF Center in February-March 2013 at Alzahra Hospital, Tabriz, Iran, who were divided into two groups as PCOS (n=15) and non-PCOS (n=15) women (controls).Results: All doses of the EPA significantly induced PPARg mRNA gene expression level as compared to the control recombinant follicle stimulating hormone (rFSH) alone condition. High doses of EPA in the presence of rFSH produced a stimulatory effect on expression level of PPARg (2.15-fold, P=0.001) and a suppressive effect (0.56-fold, P=0.01) on the expression level of CYP-19, only in the PCOS GC.Conclusion: EPA and FSH signaling pathway affect differentially on the gene expression levels of PPARg and CYP-19 in PCOS GC. Altered FSH-induced PPARg activity in PCOS GC may modulate the CYP-19 gene expression in response to EPA, and possibly modulates the subsequent steroidogenesis of these cells.

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APA: Copy

ZAREEI, MINA, SHAHNAZI, VAHIDEH, FAYEZI, SHABNAM, DARABI, MARYAM, MEHRZAD SADAGHIANI, MAHZAD, DARABI, MASOUD, KHANI, SAJJAD, & NOURI, MOHAMMAD. (2015). EXPRESSION LEVELS OF PPAR? AND CYP-19 IN POLYCYSTIC OVARIAN SYNDROME PRIMARY GRANULOSA CELLS: INFLUENCE OF ω-3 FATTY ACID. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 9(2), 197-204. SID. https://sid.ir/paper/305811/en

Vancouver: Copy

ZAREEI MINA, SHAHNAZI VAHIDEH, FAYEZI SHABNAM, DARABI MARYAM, MEHRZAD SADAGHIANI MAHZAD, DARABI MASOUD, KHANI SAJJAD, NOURI MOHAMMAD. EXPRESSION LEVELS OF PPAR? AND CYP-19 IN POLYCYSTIC OVARIAN SYNDROME PRIMARY GRANULOSA CELLS: INFLUENCE OF ω-3 FATTY ACID. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY[Internet]. 2015;9(2):197-204. Available from: https://sid.ir/paper/305811/en

IEEE: Copy

MINA ZAREEI, VAHIDEH SHAHNAZI, SHABNAM FAYEZI, MARYAM DARABI, MAHZAD MEHRZAD SADAGHIANI, MASOUD DARABI, SAJJAD KHANI, and MOHAMMAD NOURI, “EXPRESSION LEVELS OF PPAR? AND CYP-19 IN POLYCYSTIC OVARIAN SYNDROME PRIMARY GRANULOSA CELLS: INFLUENCE OF ω-3 FATTY ACID,” INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, vol. 9, no. 2, pp. 197–204, 2015, [Online]. Available: https://sid.ir/paper/305811/en

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