Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

444
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

223
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

1

Information Journal Paper

Title

INCREASED LITTER SIZE AND SUCKLING INTENSITY STIMULATE MRNA OF RFAMIDE-RELATED PEPTIDE IN RATS

Author(s)

 NOROOZI ATEFEH | JAFARZADEH SHIRAZI MOHAMMAD REZA | TAMADON AMIN | MOGHADAM ALI | NIAZI ALI | Issue Writer Certificate 

Pages

  380-386

Abstract

 Background: RFamide-related peptide-3 (RFRP-3) inhibits gonadotropin releasing hormone (GnRH) and luteinizing hormone (LH) secretion in RATs. This study evaluates the effects of litter size and SUCKLING INTENSITY on RFRP MRNA expression in the DORSOMEDIAL HYPOTHALAMIC NUCLEUS (DMH) of RATs.Materials and Methods: A total of 32 pregnant and 4 non-lactating ovariectomized (control group) Sprague-Dawley RATs were used in this experimental study. Lactating RATs were allotted to 8 equal groups. In 3 groups, the litter size was adjusted to 5, 10, or 15 pups upon parturition. Dams were allowed to suckle their pups continuously until 8 days postpartum. In the other 3 groups, the litter size was adjusted to 5 pups following birth. These pups were separated from the dams for 6 hours on day 8 postpartum, after which the pups were allowed to suckle for 2.5, 5, or 7.5 minutes prior to killing the dams. In 2 groups, lactating RATs with 10 and 15 pups were separated from their pups for 6 hours on day 8 postpartum. In these groups, the pups were allowed to suckle their dams for 5 minutes before the dams were killed. All RATs were killed on day 8 postpartum and the DMH was removed from each RAT. We evaluated RFRP MRNA expression using realtime polymerase chain reaction (PCR).Results: The expression of RFRP MRNA in the DMH increased with increased litter size and SUCKLING INTENSITY compared to the controls. The effect of SUCKLING INTENSITY on the expression of RFRP MRNA was more pronounced compared to the litter size.Conclusion: Increased litter size and SUCKLING INTENSITY stimulated RFRP MRNA expression in the DMH which might contribute to LACTATION anestrus in RATs.

Cites

References

Cite

APA: Copy

NOROOZI, ATEFEH, JAFARZADEH SHIRAZI, MOHAMMAD REZA, TAMADON, AMIN, MOGHADAM, ALI, & NIAZI, ALI. (2015). INCREASED LITTER SIZE AND SUCKLING INTENSITY STIMULATE MRNA OF RFAMIDE-RELATED PEPTIDE IN RATS. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 9(3), 380-386. SID. https://sid.ir/paper/305826/en

Vancouver: Copy

NOROOZI ATEFEH, JAFARZADEH SHIRAZI MOHAMMAD REZA, TAMADON AMIN, MOGHADAM ALI, NIAZI ALI. INCREASED LITTER SIZE AND SUCKLING INTENSITY STIMULATE MRNA OF RFAMIDE-RELATED PEPTIDE IN RATS. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY[Internet]. 2015;9(3):380-386. Available from: https://sid.ir/paper/305826/en

IEEE: Copy

ATEFEH NOROOZI, MOHAMMAD REZA JAFARZADEH SHIRAZI, AMIN TAMADON, ALI MOGHADAM, and ALI NIAZI, “INCREASED LITTER SIZE AND SUCKLING INTENSITY STIMULATE MRNA OF RFAMIDE-RELATED PEPTIDE IN RATS,” INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, vol. 9, no. 3, pp. 380–386, 2015, [Online]. Available: https://sid.ir/paper/305826/en

Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top