REDUCING INTER-LABORATORY DIFFERENCES BETWEEN SEMEN ANALYSES USING Z SCORE AND REGRESSION TRANSFORMATIONS

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LEUSHUIS ESTHER; WETZELS ALEX; VAN DER STEEG JAN WILLEM; STEURES PIETERNEL; BOSSUYT PATRICK M.M.; TROOYEN NETTY VAN; REPPING SJOERD; VAN DER HORST FRANS A.L.; HOMPES PETER G.A.; MOL BEN WILLEM J.; DER VEEN FULCO VAN

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Journal Paper

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Journal: International Journal of Fertility & Sterility Year:2016 | Volume:9 | Issue:4 Page(s): 534-540

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Title

REDUCING INTER-LABORATORY DIFFERENCES BETWEEN SEMEN ANALYSES USING Z SCORE AND REGRESSION TRANSFORMATIONS

Author(s)

Keywords

DIFFERENCES

SEMEN ANALYSIS

REGRESSION

STANDARDIZATION

Abstract

Background: STANDARDIZATION of the SEMEN ANALYSIS may improve reproducibility. We assessed variability between laboratories in semen analyses and evaluated whether a transformation using Z scores and REGRESSION statistics was able to reduce this variability.Materials and Methods: We performed a retrospective cohort study. We calculated between-laboratory coefficients of variation (CVB) for sperm concentration and for morphology. Subsequently, we standardized the SEMEN ANALYSIS results by calculating laboratory specific Z scores, and by using REGRESSION. We used analysis of variance for four semen parameters to assess systematic DIFFERENCES between laboratories before and after the transformations, both in the circulation samples and in the samples obtained in the prospective cohort study in the Netherlands between January 2002 and February 2004.Results: The mean CVB was 7% for sperm concentration (range 3 to 13%) and 32% for sperm morphology (range 18 to 51%). The DIFFERENCES between the laboratories were statistically significant for all semen parameters (all P<0.001). STANDARDIZATION using Z scores did not reduce the DIFFERENCES in SEMEN ANALYSIS results between the laboratories (all P<0.001).Conclusion: There exists large between-laboratory variability for sperm morphology and small, but statistically significant, between-laboratory variation for sperm concentration. STANDARDIZATION using Z scores does not eliminate between-laboratory variability.

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References

A DIAGNOSTIC METHOD FOR SUSPECTED ALZHEIMER’S DISEASE USING H(2)15O POSITRON EMISSION TOMOGRAPHY PERFUSION Z SCORE

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APA: Copy

LEUSHUIS, ESTHER, WETZELS, ALEX, VAN DER STEEG, JAN WILLEM, STEURES, PIETERNEL, BOSSUYT, PATRICK M.M., TROOYEN, NETTY VAN, REPPING, SJOERD, VAN DER HORST, FRANS A.L., HOMPES, PETER G.A., MOL, BEN WILLEM J., & DER VEEN, FULCO VAN. (2016). REDUCING INTER-LABORATORY DIFFERENCES BETWEEN SEMEN ANALYSES USING Z SCORE AND REGRESSION TRANSFORMATIONS. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 9(4), 534-540. SID. https://sid.ir/paper/305854/en

Vancouver: Copy

LEUSHUIS ESTHER, WETZELS ALEX, VAN DER STEEG JAN WILLEM, STEURES PIETERNEL, BOSSUYT PATRICK M.M., TROOYEN NETTY VAN, REPPING SJOERD, VAN DER HORST FRANS A.L., HOMPES PETER G.A., MOL BEN WILLEM J., DER VEEN FULCO VAN. REDUCING INTER-LABORATORY DIFFERENCES BETWEEN SEMEN ANALYSES USING Z SCORE AND REGRESSION TRANSFORMATIONS. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY[Internet]. 2016;9(4):534-540. Available from: https://sid.ir/paper/305854/en

IEEE: Copy

ESTHER LEUSHUIS, ALEX WETZELS, JAN WILLEM VAN DER STEEG, PIETERNEL STEURES, PATRICK M.M. BOSSUYT, NETTY VAN TROOYEN, SJOERD REPPING, FRANS A.L. VAN DER HORST, PETER G.A. HOMPES, BEN WILLEM J. MOL, and FULCO VAN DER VEEN, “REDUCING INTER-LABORATORY DIFFERENCES BETWEEN SEMEN ANALYSES USING Z SCORE AND REGRESSION TRANSFORMATIONS,” INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, vol. 9, no. 4, pp. 534–540, 2016, [Online]. Available: https://sid.ir/paper/305854/en

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