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Information Journal Paper

Title

EXPRESSION OF GHRELIN AND ITS RECEPTOR MRNA IN BOVINE OOCYTE AND CUMULUS CELLS

Pages

  335-338

Abstract

 Energy balance is regulated by GHRELIN which is a neuroendocrine modulator. GHRELIN is expressed in reproductive organs. However, the role of GHRELIN during in vitro maturation (IVM) and bovine preimplantational development is limited. The purpose of this study was to measure the expression of GHRELIN (GHRL) and its receptor growth hormone secretagogue receptor 1A (GHS-R1A) mRNA, and determine cumulus oocyte complex (COC) viability after IVM with 0, 20, 40 and 60 pM of GHRELIN. Also, pronuclear formation was recorded after in vitro fertilization (IVF). GHRL and GHS-R1A MRNA EXPRESSION in oocyte and cumulus cells (CCs) was assessed using reverse transcription-polymerase chain reaction (PCR). Oocyte and CC viability were analyzed with the fluorescein diacetate fluorochrome-trypan blue technique. Pronuclear formation was determined 18 hours after IVF with Hoechst 33342. The results demonstrated that GHRELIN mRNA is present in oocyte and CCs before and after 24 hours IVM with all treatments. GHRELIN receptor, GHS-R1A, was only detected in oocytes and CCs after 24 hours IVM with 20, 40 and 60 pM of GHRELIN. Oocyte viability was not significantly different (P=0.77) among treatments. However, CC viability was significantly lower (P=0.04) when COCs were matured with GHRELIN (77.65, 72.10, 66.32 and 46.86% for 0, 20, 40, and 60 pM of GHRELIN, respectively). The chance of two pronuclei forming were higher (P=0.03) when GHRELIN was not be added to the IVM medium. We found that GHRELIN negatively impacts CC viability and pronuclear formation.

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    APA: Copy

    SIRINI, MATIAS ANGEL, ANCHORDOQUY, JUAN PATRICIO, QUINTANA, SILVINA, FURNUS, CECILIA, RELLING, ALEJANDRO ENRIQUE, & ANCHORDOQUY, JUAN MATEO. (2019). EXPRESSION OF GHRELIN AND ITS RECEPTOR MRNA IN BOVINE OOCYTE AND CUMULUS CELLS. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 12(4), 335-338. SID. https://sid.ir/paper/306016/en

    Vancouver: Copy

    SIRINI MATIAS ANGEL, ANCHORDOQUY JUAN PATRICIO, QUINTANA SILVINA, FURNUS CECILIA, RELLING ALEJANDRO ENRIQUE, ANCHORDOQUY JUAN MATEO. EXPRESSION OF GHRELIN AND ITS RECEPTOR MRNA IN BOVINE OOCYTE AND CUMULUS CELLS. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY[Internet]. 2019;12(4):335-338. Available from: https://sid.ir/paper/306016/en

    IEEE: Copy

    MATIAS ANGEL SIRINI, JUAN PATRICIO ANCHORDOQUY, SILVINA QUINTANA, CECILIA FURNUS, ALEJANDRO ENRIQUE RELLING, and JUAN MATEO ANCHORDOQUY, “EXPRESSION OF GHRELIN AND ITS RECEPTOR MRNA IN BOVINE OOCYTE AND CUMULUS CELLS,” INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, vol. 12, no. 4, pp. 335–338, 2019, [Online]. Available: https://sid.ir/paper/306016/en

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