PROPAGATION OF HEPATITIS B VIRUS IN A RAT HEPATOMA CELL LINE STABLY TRANSFECTED WITH HUMAN ANNEXIN-V

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

JAZAYERI SEYED MOHAMMAD; DORNAN E.S.; DEPLA E.; CARMAN W.F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Hepatitis Monthly Year:2007 | Volume:7 | Issue:3 Page(s): 143-147

Download Full-Text

View:

355

Download:

347

Cites:

Information Journal Paper

Title

PROPAGATION OF HEPATITIS B VIRUS IN A RAT HEPATOMA CELL LINE STABLY TRANSFECTED WITH HUMAN ANNEXIN-V

Author(s)

JAZAYERI SEYED MOHAMMAD | DORNAN E.S. | DEPLA E. | CARMAN W.F. | Issue Writer Certificate

Keywords

HBV

IN VITRO

FTO 9.1 CELL LINE

HUMAN ANNEXIN-V

Abstract

Background and Aims: Hepatitis B virus (HBV) displays a distinct hepatotropism and a narrow host range in vivo. However, very little is known about the interaction of HBV with its host cells, mainly because of difficulties in the development of suitable tissue culture system. We present here confirmatory evidence of a putative role of annexin-V in HBV infection.Methods: HBV from both human sera and from culture supernatants from HepG2 2.15 cells were used to infect FTO9.1 cells (a rat hepatoma cell line transfected with a construct containing HUMAN ANNEXIN-V). Cells and culture supernatants were assayed at various times post-infection by immunofluorescent microscopy (HBcAg staining in nucleus), and by HBV cccDNA-specific PCR. Supernatants from these initially infected cells were then used to infect fresh FTO9.1 cells with a similar outcome to primary infection.Results: Core and surface gene PCRs were positive on days 2, 5 and following transfer experiments. cccDNA-specific PCR confirmed internalisation of the virus into the nucleus. HBcAg fluorescence showed nuclear staining on days 2, 5 and following transfer experiments. Addition of recombinant annexin-V and DMSO to the cell culture medium resulted in a greater efficiency of infection. Later washes were negative for HBV-DNA, ruling out contamination of the cells by external HBV particles.Conclusions: This cell line does appear to be useful in the study of the early stages of HBV infection, but requires further evaluation.

Cites

No record.

References

No record.

Cite

APA: Copy

JAZAYERI, SEYED MOHAMMAD, DORNAN, E.S., DEPLA, E., & CARMAN, W.F.. (2007). PROPAGATION OF HEPATITIS B VIRUS IN A RAT HEPATOMA CELL LINE STABLY TRANSFECTED WITH HUMAN ANNEXIN-V. HEPATITIS MONTHLY, 7(3), 143-147. SID. https://sid.ir/paper/306135/en

Vancouver: Copy

JAZAYERI SEYED MOHAMMAD, DORNAN E.S., DEPLA E., CARMAN W.F.. PROPAGATION OF HEPATITIS B VIRUS IN A RAT HEPATOMA CELL LINE STABLY TRANSFECTED WITH HUMAN ANNEXIN-V. HEPATITIS MONTHLY[Internet]. 2007;7(3):143-147. Available from: https://sid.ir/paper/306135/en

IEEE: Copy

SEYED MOHAMMAD JAZAYERI, E.S. DORNAN, E. DEPLA, and W.F. CARMAN, “PROPAGATION OF HEPATITIS B VIRUS IN A RAT HEPATOMA CELL LINE STABLY TRANSFECTED WITH HUMAN ANNEXIN-V,” HEPATITIS MONTHLY, vol. 7, no. 3, pp. 143–147, 2007, [Online]. Available: https://sid.ir/paper/306135/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops