مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Cites:

1

Information Journal Paper

Title

PROTECTIVE EFFECT OF INTERMITTENT CLAMPING OF THE PORTAL TRIAD IN THE RAT LIVER ON LIVER ISCHEMIA-REPERFUSION INJURY

Pages

  445-451

Abstract

 Background: Intermittent clamping (IC) of the portal triad is an effective method of protecting the liver from ischemia-reperfusion injury (IR). In clinical practice, this method is employed during a resection, but its mechanism is still not clear.Objectives: To evaluate the effect of IC on rat liver and determine its mechanisms.Materials and Methods: Wistar rats were submitted to 60-min IC (cycles of 12-min clamping followed by 4-min reperfusion), and the samples were collected after 1, 6, and 72 hrs of reperfusion. We determined the serum activity of alanine aminotransferase (ALT), and measured the concentration of TNF-a, malondialdehyde (MDA) and myeloperoxidase (MPO) in liver homogenates. The APOPTOSIS of hepatocytes was evaluated immunohistochemically.Results: When compared to the IR rats, the activity of ALT decreased in the IC group in all periods of observation (the highest decrease of ~48% after 1 hr of reperfusion).When compared to the IR group, a statistically significant decrease (p<0.05) in the TNF-a concentration (~33%) in the IC rats occurred only after 1 hr of reperfusion, and it was accompanied by a decrease in the MPO concentration after 1 and 6 hrs of reperfusion.IC reduces the effects of reactive oxygen species (ROS) activity, which has been confirmed by a statistically significant decrease in MDA concentration by 25%–35% in all studied periods. The limitation of hepatocytes APOPTOSIS due to IC occurs in the early (~26%; p<0.05) and late (~45%; p<0.01) phases of reperfusion.Conclusions: The use of IC in early phase of reperfusion brings about a decrease in TNF-a release, which can be related to liver injury due to neutrophil infiltration and apoptotic cell reduction. It seems that the reduction of lipid peroxidation may also limit the liver injury.

Cites

References

Cite

APA: Copy

HELEWSKI, KRZYSZTOF, KOWALCZYK ZIOMEK, GRAZYNA, CZECIOR, EUGENIUSZ, WYROBIEC, GRZEGORZ, HARABIN SLOWINSKA, MARZENA, JUSZKO PIEKUT, MALGORZATA, BRACZKOWSKA, BOGUMILA, & JOSKO, JADWIGA. (2011). PROTECTIVE EFFECT OF INTERMITTENT CLAMPING OF THE PORTAL TRIAD IN THE RAT LIVER ON LIVER ISCHEMIA-REPERFUSION INJURY. HEPATITIS MONTHLY, 11(6 (35)), 445-451. SID. https://sid.ir/paper/306412/en

Vancouver: Copy

HELEWSKI KRZYSZTOF, KOWALCZYK ZIOMEK GRAZYNA, CZECIOR EUGENIUSZ, WYROBIEC GRZEGORZ, HARABIN SLOWINSKA MARZENA, JUSZKO PIEKUT MALGORZATA, BRACZKOWSKA BOGUMILA, JOSKO JADWIGA. PROTECTIVE EFFECT OF INTERMITTENT CLAMPING OF THE PORTAL TRIAD IN THE RAT LIVER ON LIVER ISCHEMIA-REPERFUSION INJURY. HEPATITIS MONTHLY[Internet]. 2011;11(6 (35)):445-451. Available from: https://sid.ir/paper/306412/en

IEEE: Copy

KRZYSZTOF HELEWSKI, GRAZYNA KOWALCZYK ZIOMEK, EUGENIUSZ CZECIOR, GRZEGORZ WYROBIEC, MARZENA HARABIN SLOWINSKA, MALGORZATA JUSZKO PIEKUT, BOGUMILA BRACZKOWSKA, and JADWIGA JOSKO, “PROTECTIVE EFFECT OF INTERMITTENT CLAMPING OF THE PORTAL TRIAD IN THE RAT LIVER ON LIVER ISCHEMIA-REPERFUSION INJURY,” HEPATITIS MONTHLY, vol. 11, no. 6 (35), pp. 445–451, 2011, [Online]. Available: https://sid.ir/paper/306412/en

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