ENHANCEMENT OF IMMUNE RESPONSES BY CO-DELIVERY OF CCL19/MIP-3BETA CHEMOKINE PLASMID WITH HCV CORE DNA/PROTEIN IMMUNIZATION

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HARTOONIAN CHRISTINE; SEPEHRIZADEH ZARGHAM; TABATABAI YAZDI MOJTABA; JANG YONG SUK; LANGROUDI LIDA; AMIR KALVANAGH PARISA; NEGAHDARI BABAK; KARAMI ALI; EBTEKAR MASSOUMEH; AZADMANESH KAYHAN

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Journal: Hepatitis Monthly Year:2014 | Volume:14 | Issue:3 Page(s): 1-9

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Title

ENHANCEMENT OF IMMUNE RESPONSES BY CO-DELIVERY OF CCL19/MIP-3BETA CHEMOKINE PLASMID WITH HCV CORE DNA/PROTEIN IMMUNIZATION

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Keywords

CHEMOKINE CCL19

HEPATITIS C

HEPATITIS C ANTIGENS

ADJUVANTS

Abstract

Background: Using molecular ADJUVANTS offers an attractive strategy to augment DNA vaccine-mediated immune responses. Several studies have revealed that an efficient HCV vaccine model should be able to induce both humoral and cell mediated immune responses targeting the conserved regions of the virus to circumvent the immune escape mutants. The beta chemokine Macrophage Inflammatory Protein 3-beta (MIP-3beta) is a key modulator of dendritic cells (DCs) and T-cells interaction, functions during immune response induction and is secreted specifically by cells in the lymphoid tissues.Objectives: In the present study, we questioned whether co-administration of MIP-3beta gene could enhance the immune responses to HCV core in DNA vaccination.Materials and Methods: Expression and biological activity of MIP-3beta expressing plasmid were evaluated by ELISA and transwell migration assays, respectively. HCV core DNA vaccine±plasmid expressing MIP-3beta were electroporated subcutaneously to the front foot pads of BALB/c mice on days 0 and 14, and HCV core protein booster was applied to all core-DNA-vaccine received mice on the day 28. Both cell mediated immunity (proliferation, IFN-g and IL-4 cytokine release, IFN-g ELISpot and cytotoxic Granzyme B release assays) and humoral immune responses (total IgG and IgG2a/IgG1 subtyping) were evaluated ten days after final immunization.Results: Mice covaccinated with MIP-3beta elicited an enhanced Th1 biased systemic immune response as evidenced by higher IFN-g/IL-4 and anti-core IgG2a/IgG1 ratio, lymphoproliferation, strong cytolytic GrzB release and enhanced population of IFN-g producing immunocytes. Likewise, the humoral immune response assumed as the total anti-core IgG level was augmented by MIP-3beta co-delivery.Conclusions: These results exhibited the immuno potentiator effects of MIP-3beta plasmid when coadministrated with the HCV core DNA vaccine. Complimentary studies integrating MIP-3beta as a genetic adjuvant in HCV-core-DNA vaccination models are warranted.

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APA: Copy

HARTOONIAN, CHRISTINE, SEPEHRIZADEH, ZARGHAM, TABATABAI YAZDI, MOJTABA, JANG, YONG SUK, LANGROUDI, LIDA, AMIR KALVANAGH, PARISA, NEGAHDARI, BABAK, KARAMI, ALI, EBTEKAR, MASSOUMEH, & AZADMANESH, KAYHAN. (2014). ENHANCEMENT OF IMMUNE RESPONSES BY CO-DELIVERY OF CCL19/MIP-3BETA CHEMOKINE PLASMID WITH HCV CORE DNA/PROTEIN IMMUNIZATION. HEPATITIS MONTHLY, 14(3), 1-9. SID. https://sid.ir/paper/306576/en

Vancouver: Copy

HARTOONIAN CHRISTINE, SEPEHRIZADEH ZARGHAM, TABATABAI YAZDI MOJTABA, JANG YONG SUK, LANGROUDI LIDA, AMIR KALVANAGH PARISA, NEGAHDARI BABAK, KARAMI ALI, EBTEKAR MASSOUMEH, AZADMANESH KAYHAN. ENHANCEMENT OF IMMUNE RESPONSES BY CO-DELIVERY OF CCL19/MIP-3BETA CHEMOKINE PLASMID WITH HCV CORE DNA/PROTEIN IMMUNIZATION. HEPATITIS MONTHLY[Internet]. 2014;14(3):1-9. Available from: https://sid.ir/paper/306576/en

IEEE: Copy

CHRISTINE HARTOONIAN, ZARGHAM SEPEHRIZADEH, MOJTABA TABATABAI YAZDI, YONG SUK JANG, LIDA LANGROUDI, PARISA AMIR KALVANAGH, BABAK NEGAHDARI, ALI KARAMI, MASSOUMEH EBTEKAR, and KAYHAN AZADMANESH, “ENHANCEMENT OF IMMUNE RESPONSES BY CO-DELIVERY OF CCL19/MIP-3BETA CHEMOKINE PLASMID WITH HCV CORE DNA/PROTEIN IMMUNIZATION,” HEPATITIS MONTHLY, vol. 14, no. 3, pp. 1–9, 2014, [Online]. Available: https://sid.ir/paper/306576/en

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