OCCULT HEPATITIS C VIRUS INFECTION IN CANDIDATES FOR LIVER TRANSPLANT WITH CRYPTOGENIC CIRRHOSIS

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KEYVANI HOSSEIN; BOKHARAEI SALIM FARAH; MONAVARI SEYED HAMIDREZA; ESGHAEI MARYAM; NASSIRI TOOSI MOHSSEN; FAKHIM SHAHIN; SADIGH ZOHREH AZITA; ALAVIAN SEYED MOAYED

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Journal: Hepatitis Monthly Year:2013 | Volume:13 | Issue:8 Page(s): 1-7

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Title

OCCULT HEPATITIS C VIRUS INFECTION IN CANDIDATES FOR LIVER TRANSPLANT WITH CRYPTOGENIC CIRRHOSIS

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Keywords

HEPATITIS C VIRUS; OCCULT INFECTION; PERIPHERAL BLOOD MONONUCLEAR CELLS; CRYPTOGENIC; CIRRHOSIS; LIVER TRANSPLANTATION

Abstract

Background: Occult hepatitis C virus (HCV) infection is a new entity described by the presence of HCV-RNA in liver biopsy and/or peripheral blood mononuclear cell (PBMC) specimens, and undetectable levels or absence of HCV-RNA and in the absence or presence of anti HCV antibodies in plasma by current laboratory methods.Objectives: To evaluate the detection of HCV-RNA in PBMC specimens of the liver transplant candidates with cryptogenic cirrhosis by reverse transcriptase-nested polymerase chain reaction (RT-nested PCR).Patients and Methods: From November 2007 to March 2013, 45 patients from Liver Transplant Center of Imam Khomeini Hospital, Tehran, were enrolled in this cross sectional study. PBMC specimens were separated from the peripheral blood sample. After extraction of RNA from plasma and PBMC specimens, HCV-RNA status was tested by RT-nested PCR. The 5′-untranslated region (5′-UTR) genotyping of HCV-RNA amplified from PBMC specimens was performed by a standard methodology with the INNO-LiPATM HCV II kit. The PCR products of 5′-UTR were sequenced after cloning into the pJET1.2 / blunt cloning vector.Results: Of 45 patients, 4 (8.9% [95% CI: 4.4-15.6]) had detectable genomic HCV-RNA in their PBMC specimens. HCV genotypes were determined in the PBMCs of these subjects showed that 2 (50.0%) subjects with occult HCV infection had HCV subtype 3a, and 2 (50.0%) had HCV subtype 1b.Conclusions: This study found that 8.9 % of the Iranian candidates for liver transplant with cryptogenic cirrhosis had occult HCV infection. Therefore, designing prospective studies focusing on the diagnosis of occult HCV infection in these subjects prior to liver transplantation could be valuable.

Cites

MOLECULAR EPIDEMIOLOGY OF KAPOSI’S SARCOMA-ASSOCIATED HERPES VIRUS, AND RISK FACTORS IN HIV-INFECTED PATIENTS IN TEHRAN, 2014

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APA: Copy

KEYVANI, HOSSEIN, BOKHARAEI SALIM, FARAH, MONAVARI, SEYED HAMIDREZA, ESGHAEI, MARYAM, NASSIRI TOOSI, MOHSSEN, FAKHIM, SHAHIN, SADIGH, ZOHREH AZITA, & ALAVIAN, SEYED MOAYED. (2013). OCCULT HEPATITIS C VIRUS INFECTION IN CANDIDATES FOR LIVER TRANSPLANT WITH CRYPTOGENIC CIRRHOSIS. HEPATITIS MONTHLY, 13(8), 1-7. SID. https://sid.ir/paper/306683/en

Vancouver: Copy

KEYVANI HOSSEIN, BOKHARAEI SALIM FARAH, MONAVARI SEYED HAMIDREZA, ESGHAEI MARYAM, NASSIRI TOOSI MOHSSEN, FAKHIM SHAHIN, SADIGH ZOHREH AZITA, ALAVIAN SEYED MOAYED. OCCULT HEPATITIS C VIRUS INFECTION IN CANDIDATES FOR LIVER TRANSPLANT WITH CRYPTOGENIC CIRRHOSIS. HEPATITIS MONTHLY[Internet]. 2013;13(8):1-7. Available from: https://sid.ir/paper/306683/en

IEEE: Copy

HOSSEIN KEYVANI, FARAH BOKHARAEI SALIM, SEYED HAMIDREZA MONAVARI, MARYAM ESGHAEI, MOHSSEN NASSIRI TOOSI, SHAHIN FAKHIM, ZOHREH AZITA SADIGH, and SEYED MOAYED ALAVIAN, “OCCULT HEPATITIS C VIRUS INFECTION IN CANDIDATES FOR LIVER TRANSPLANT WITH CRYPTOGENIC CIRRHOSIS,” HEPATITIS MONTHLY, vol. 13, no. 8, pp. 1–7, 2013, [Online]. Available: https://sid.ir/paper/306683/en

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