MOLECULAR EPIDEMIOLOGY OF KAPOSI’S SARCOMA-ASSOCIATED HERPES VIRUS, AND RISK FACTORS IN HIV-INFECTED PATIENTS IN TEHRAN, 2014

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HESAMIZADEH KHASHAYAR; KEYVANI HOSSEIN; BOKHARAEI SALIM FARAH; MONAVARI SEYED HAMIDREZA; ESGHAEI MARYAM; JAHANBAKHSH SEFIDI FATEMEH

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Journal: Iranian Red Crescent Medical Journal Year:2016 | Volume:18 | Issue:11 Page(s): 0-0

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Title

MOLECULAR EPIDEMIOLOGY OF KAPOSI’S SARCOMA-ASSOCIATED HERPES VIRUS, AND RISK FACTORS IN HIV-INFECTED PATIENTS IN TEHRAN, 2014

Author(s)

Keywords

HERPESVIRUS 8

HUMAN

HIV

SARCOMA

KAPOSI

NESTED PCR

Abstract

Background: KAPOSI’s SARCOMA (KS) remains the most common alignancy among HIV-infected patients. HUMAN herpes virus type- 8 (HHV-8) is regarded as the infectious etiological agent of KAPOSI’s SARCOMA (KSHV). Diagnostic procedures associated with KSHV are not routinely performed in HIV-infected subjects.Objectives: The main objective of this study is to obtain information on KSHV epidemiology in Iranian HIV-infected individuals.Patients and Methods: In the present cross-sectional study, 109 patients with established HIV infection, who visited a governmental and referral center for HIV screening in Tehran (Tehran west health center (TWHC)) between May 2014 and July 2015 were enrolled according to the convenience sample strategy. After peripheral blood collection, isolation of plasma and peripheral blood mononuclear cell (PBMC) compartments, DNA extraction was performed. KSHV DNA was analyzed by nested polymerase chain reaction (nested PCR) using primers from ORF-26 (virus minor capsid).Results: Among all 109 HIV-infected patients, 67 (61.5%) were male, with an age range of 2 - 64 years (mean±standard deviation 35.8±13.3). KSHV DNA was found in PBMC and plasma samples of six (5.5%) and four (3.6%) patients, respectively.Conclusions: This study revealed a considerable prevalence of KSHV DNA, during latent and lytic phases, among HIV-infected patients.Risk factors for KSHV infection acquisition and concurrent.0+infection with HIV were also evaluated. Diagnosis of KSHV in the group could be helpful for prognosis of KAPOSI’s SARCOMA and clinical management.

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APA: Copy

HESAMIZADEH, KHASHAYAR, KEYVANI, HOSSEIN, BOKHARAEI SALIM, FARAH, MONAVARI, SEYED HAMIDREZA, ESGHAEI, MARYAM, & JAHANBAKHSH SEFIDI, FATEMEH. (2016). MOLECULAR EPIDEMIOLOGY OF KAPOSI’S SARCOMA-ASSOCIATED HERPES VIRUS, AND RISK FACTORS IN HIV-INFECTED PATIENTS IN TEHRAN, 2014. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), 18(11), 0-0. SID. https://sid.ir/paper/293458/en

Vancouver: Copy

HESAMIZADEH KHASHAYAR, KEYVANI HOSSEIN, BOKHARAEI SALIM FARAH, MONAVARI SEYED HAMIDREZA, ESGHAEI MARYAM, JAHANBAKHSH SEFIDI FATEMEH. MOLECULAR EPIDEMIOLOGY OF KAPOSI’S SARCOMA-ASSOCIATED HERPES VIRUS, AND RISK FACTORS IN HIV-INFECTED PATIENTS IN TEHRAN, 2014. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ)[Internet]. 2016;18(11):0-0. Available from: https://sid.ir/paper/293458/en

IEEE: Copy

KHASHAYAR HESAMIZADEH, HOSSEIN KEYVANI, FARAH BOKHARAEI SALIM, SEYED HAMIDREZA MONAVARI, MARYAM ESGHAEI, and FATEMEH JAHANBAKHSH SEFIDI, “MOLECULAR EPIDEMIOLOGY OF KAPOSI’S SARCOMA-ASSOCIATED HERPES VIRUS, AND RISK FACTORS IN HIV-INFECTED PATIENTS IN TEHRAN, 2014,” IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), vol. 18, no. 11, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/293458/en

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