A COMPARATIVE EVOLUTION STUDY OF EAE GENES OF THE LEE LOCI IN E.COLI STRAINS IN IRAN WITH DIFFERENT COUNTRIES

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ASADI KARAM M.R.; ASLANI M.M.; HABIBI M.; OLOUMI MANA

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Journal: Daneshvar Medicine: Basic and Clinical Research Journal Year:2007 | Volume:14 | Issue:69 Page(s): 9-18

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Title

A COMPARATIVE EVOLUTION STUDY OF EAE GENES OF THE LEE LOCI IN E.COLI STRAINS IN IRAN WITH DIFFERENT COUNTRIES

Author(s)

ASADI KARAM M.R. | ASLANI M.M. | HABIBI M. | OLOUMI MANA | Issue Writer Certificate

Keywords

DIARRHEAQ3

PATHOGENECITY ISLANDQ3

GENEQ2

TYPEQ2

SEROGROUPQ3

INSERTION SITEQ3

Abstract

Introduction: Diarrhoea is a major public health problem especially in developing countries, and despite the intensive efforts made in the field of prevention and treatment, it remains one of the biggest child –killers requiring further research. Enteropathogenic and Enterohemorrhagic Escherichia coli are leading cause of infantile DIARRHEA in developing countries. Eae (intimin) GENE located on LEE (locus of enterocyte effacement) PATHOGENECITY ISLAND. eae in EPEC and EHEC pathotypes is responsible for attachig and effacing lesions in host intestinal epithelium. Nterminal part of the GENE is conserved whereas the C-terminal is variable, resulting in heterogeneity of the GENE in different isolates. the INSERTION SITEs of LEE are SelC, PheU and PheV. the INSERTION SITEs of LEE is depending on the clonal phylogeny of the E.coli strains.Materials & Methods: E.coli Strains used in this study were isolated from children with hospitalized in Tehran. The presence of eae GENE, typing of the GENE and determination of INSERTION SITE of LEE PATHOGENECITY ISLANDs was ascertained by PCR using primers in Table1. PCR products were further confirmed and subtyped by resteriction enzyme PstI. Detection of Stx GENEs was ascertained by PCR using primers in Table1.the isolates were TYPEd with EPEC antisera.Results: In this study eae GENE was detected in 11.8% of E.coli isolates and further typing showed that 15.7% were E1, 2.6% E2 , 23.6% F1 , 10.5% F2 , and 2.6% were G respectely. in this study 42.7% of isolates carried an untypiable intimin. Serology identified 21(42.1%) EPEC isolates but none of the isolates possessed Stx GENEs, 57.9% of isolates was belong to attachingeffacing Escherichia coli (AEEC). Among the isolates, SelC (47.61%) and PheU (42.87%) were the sites mainly used for insertion of LEE and PheV was accupied only in two (9.52%) isolates. Discussion: Our data showed that in accordance with results from similar studies reported from other countries, presence rates of eae GENE and typing of this GENE is different and the difference is not significant. in this study 42.7 % of isolates carried an untypiable intimin that is similar to results from other countries. the INSERTION SITEs for intimin TYPE E and G was SelC, but TYPE F was inserted in both PheU and PheV sites, similar to reportes for isolates in other part of the world.

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APA: Copy

ASADI KARAM, M.R., ASLANI, M.M., HABIBI, M., & OLOUMI, MANA. (2007). A COMPARATIVE EVOLUTION STUDY OF EAE GENES OF THE LEE LOCI IN E.COLI STRAINS IN IRAN WITH DIFFERENT COUNTRIES. DANESHVAR MEDICINE, 14(69), 9-18. SID. https://sid.ir/paper/30964/en

Vancouver: Copy

ASADI KARAM M.R., ASLANI M.M., HABIBI M., OLOUMI MANA. A COMPARATIVE EVOLUTION STUDY OF EAE GENES OF THE LEE LOCI IN E.COLI STRAINS IN IRAN WITH DIFFERENT COUNTRIES. DANESHVAR MEDICINE[Internet]. 2007;14(69):9-18. Available from: https://sid.ir/paper/30964/en

IEEE: Copy

M.R. ASADI KARAM, M.M. ASLANI, M. HABIBI, and MANA OLOUMI, “A COMPARATIVE EVOLUTION STUDY OF EAE GENES OF THE LEE LOCI IN E.COLI STRAINS IN IRAN WITH DIFFERENT COUNTRIES,” DANESHVAR MEDICINE, vol. 14, no. 69, pp. 9–18, 2007, [Online]. Available: https://sid.ir/paper/30964/en

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