RETINAL PIGMENT EPITHELIUM CULTURE; A POTENTIAL SOURCE OF RETINAL STEM CELLS

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AKRAMI HASAN; SOHEYLI Z.S.; KHALOUGHI K.; AHMADIEH H.; REZAEI KANAVI M.; SAMIEI SH.; DAVARI M.; GHADERI SH.; SANEEI JAHROMI F.

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Journal Paper

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Journal: Journal of Ophthalmic and Vision Research Year:2009 | Volume:4 | Issue:3 Page(s): 134-141

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Title

RETINAL PIGMENT EPITHELIUM CULTURE; A POTENTIAL SOURCE OF RETINAL STEM CELLS

Author(s)

Keywords

RETINAL PIGMENT EPITHELIUM

STEM CELLS

Abstract

Purpose: To establish human retinal pigment epithelial (RPE) cell culture as a source for cell replacement therapy in ocular diseases. Methods: Human cadaver globes were used to isolate RPE cells. Each globe was cut into several pieces of a few millimeters in size. After removing the sclera and choroid, remaining tissues were washed in phosphate buffer saline and RPE cells were isolated using dispase enzyme solution and cultured in Dulbecco’s Modified Eagle’s Medium: Nutrient Mixture F-12 supplemented with 10% fetal calf serum. Results: Primary cultures of RPE cells were established and spheroid colonies related to progenitor/stem cells developed in a number of cultures. The colonies included purely pigmented or mixed pigmented and non-pigmented cells. After multiple cellular pass-ages, several types of photoreceptors and neural-like cells were detected morpho-logically. Conclusion: Cellular plasticity in RPE cell cultures revealed promising results in terms of generation of stem/progenitor cells from human RPE cells. Whether the spheroids and neural-like retinal cells were directly derived from retinal STEM CELLS or offspring of trans-differentiating or de-differentiating RPE cells remains to be answered.

Cites

EDITORIAL; HISCOTT AND SHERIDAN: RETINAL PIGMENT EPITHELIUM DERIVED STEM CELLS

References

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APA: Copy

AKRAMI, HASAN, SOHEYLI, Z.S., KHALOUGHI, K., AHMADIEH, H., REZAEI KANAVI, M., SAMIEI, SH., DAVARI, M., GHADERI, SH., & SANEEI JAHROMI, F.. (2009). RETINAL PIGMENT EPITHELIUM CULTURE; A POTENTIAL SOURCE OF RETINAL STEM CELLS. JOURNAL OF OPHTHALMIC AND VISION RESEARCH, 4(3), 134-141. SID. https://sid.ir/paper/311376/en

Vancouver: Copy

AKRAMI HASAN, SOHEYLI Z.S., KHALOUGHI K., AHMADIEH H., REZAEI KANAVI M., SAMIEI SH., DAVARI M., GHADERI SH., SANEEI JAHROMI F.. RETINAL PIGMENT EPITHELIUM CULTURE; A POTENTIAL SOURCE OF RETINAL STEM CELLS. JOURNAL OF OPHTHALMIC AND VISION RESEARCH[Internet]. 2009;4(3):134-141. Available from: https://sid.ir/paper/311376/en

IEEE: Copy

HASAN AKRAMI, Z.S. SOHEYLI, K. KHALOUGHI, H. AHMADIEH, M. REZAEI KANAVI, SH. SAMIEI, M. DAVARI, SH. GHADERI, and F. SANEEI JAHROMI, “RETINAL PIGMENT EPITHELIUM CULTURE; A POTENTIAL SOURCE OF RETINAL STEM CELLS,” JOURNAL OF OPHTHALMIC AND VISION RESEARCH, vol. 4, no. 3, pp. 134–141, 2009, [Online]. Available: https://sid.ir/paper/311376/en

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