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Cites:

1

Information Journal Paper

Title

EXTRACELLULAR CASPASE-8 DEPENDENT APOPTOSIS ON HELA CANCER CELLS AND MRC-5 NORMAL CELLS BY ICD-85 (VENOM DERIVED PEPTIDES)

Pages

  194-202

Abstract

 Background: Our previous studies revealed an inhibitory effect of ICD-85 (venom derived peptides) on MDA-MB231 and HL-60 cell lines, through induction of APOPTOSIS. The purpose of this study was to investigate APOPTOSIS-induced mechanism on HeLa and MRC-5 cells by ICD-85 through activation of CASPASE-8.Methods: Cell viability, cytosolic enzyme Lactate Dehydrogenase (LDH) and cell morphology were assessed under unexposed and ICD-85 exposed conditions.CASPASE-8 activity was assayed by CASPASE-8 colorimetric assay Kit.Results: The results show that Inhibitory Concentration 50% (IC50) value of ICD-85 for HELA CELLS at 24 h was estimated and found to be 25.32±2.15mg/ml.Furthermore, treatment of HELA CELLS with ICD-85 at concentrations of 1.6×10 and 2.6´10mg/ml did not significantly increase LDH release. Morphological changes in HELA CELLS on treatment with ICD-85 compared with untreated HELA CELLS consistent with an apoptotic mechanism of cell death, such as cell shrinkage which finally results in the generation of apoptotic bodies. However, when MRC-5 cells were exposed to ICD-85, no significant changes in cell morphology and LDH were observed at concentrations below 2.6´10mg/ml. Also, the APOPTOSIS-induction mechanism by ICD-85 on HELA CELLS was found through activation of CASPASE-8 and the activity of CASPASE-8 in HELA CELLS was 1.5 folds more than its activity on MRC-5 cells.Conclusion: Therefore, the APOPTOSIS-induced mechanisms by ICD-85 are through activation of CASPASE-8 and concerning the least cytotoxic effect on MRC-5 cells, ICD-85 may be used as anticancer compound to inhibit growth of cancer cells.

Cites

References

Cite

APA: Copy

ZARE MIRAKABADI, ABBAS, & SARZAEEM, ALI. (2012). EXTRACELLULAR CASPASE-8 DEPENDENT APOPTOSIS ON HELA CANCER CELLS AND MRC-5 NORMAL CELLS BY ICD-85 (VENOM DERIVED PEPTIDES). INTERNATIONAL JOURNAL OF CANCER MANAGEMENT (IRANIAN JOURNAL OF CANCER PREVENTION), 5(4), 194-202. SID. https://sid.ir/paper/312570/en

Vancouver: Copy

ZARE MIRAKABADI ABBAS, SARZAEEM ALI. EXTRACELLULAR CASPASE-8 DEPENDENT APOPTOSIS ON HELA CANCER CELLS AND MRC-5 NORMAL CELLS BY ICD-85 (VENOM DERIVED PEPTIDES). INTERNATIONAL JOURNAL OF CANCER MANAGEMENT (IRANIAN JOURNAL OF CANCER PREVENTION)[Internet]. 2012;5(4):194-202. Available from: https://sid.ir/paper/312570/en

IEEE: Copy

ABBAS ZARE MIRAKABADI, and ALI SARZAEEM, “EXTRACELLULAR CASPASE-8 DEPENDENT APOPTOSIS ON HELA CANCER CELLS AND MRC-5 NORMAL CELLS BY ICD-85 (VENOM DERIVED PEPTIDES),” INTERNATIONAL JOURNAL OF CANCER MANAGEMENT (IRANIAN JOURNAL OF CANCER PREVENTION), vol. 5, no. 4, pp. 194–202, 2012, [Online]. Available: https://sid.ir/paper/312570/en

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